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Literature summary extracted from

  • Camadro, J.M.; Labbe, P.
    Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides (1996), J. Biol. Chem., 271, 9120-9128.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4
-
 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.4 K424E inactive Saccharomyces cerevisiae
1.3.3.4 L422P inactive Saccharomyces cerevisiae
1.3.3.4 L422P/K424E double mutant, inactive Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.4 additional information inhibited by diphenylether herbicides Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 0.00002
-
 protoporphyrinogen-IX
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.4 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 59670
-
 calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2 Saccharomyces cerevisiae penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.4 0.73
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Saccharomyces cerevisiae protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Saccharomyces cerevisiae protoporphyrin-IX + 3 H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 flavin
-
 Saccharomyces cerevisiae