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Literature summary extracted from

  • Yamamoto, M.; Nomura, M.; Ohsawa, H.; Maruo, B.
    Identification of a temperature-sensitive asparaginyl-transfer ribonucleic acid synthetase mutant of Escherichia coli (1977), J. Bacteriol., 132, 127-131.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.22 Escherichia coli
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temperature-sensitive mutant HO202
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.22 ATP + L-asparagine + tRNAAsn
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Escherichia coli AMP + diphosphate + L-asparaginyl-tRNAAsn
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?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.22 additional information
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mutant enzyme has no activity at 42°C and very weak activity at 30°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.22 42
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very unstable at Escherichia coli