BRENDA - Enzyme Database show

The enzymic conversion of protoporphyrinogen IX to protoporphyrin IX. Protoporphyrinogen oxidase activity in mitochondrial extracts of Saccharomyces cerevisiae

Poulson, R.; Polglase, W.J.; J. Biol. Chem. 250, 1269-1274 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.3.4
cysteine
-
Saccharomyces cerevisiae
1.3.3.4
dithiothreitol
-
Saccharomyces cerevisiae
1.3.3.4
glutathione
-
Saccharomyces cerevisiae
General Stability
EC Number
General Stability
Organism
1.3.3.4
sensitive towards trypsin and chymotrypsin
Saccharomyces cerevisiae
1.3.3.4
stable on dialysis
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.3.4
arsenite
slight inhibition
Saccharomyces cerevisiae
1.3.3.4
Co2+
inhibitory
Saccharomyces cerevisiae
1.3.3.4
Cu2+
inhibitory
Saccharomyces cerevisiae
1.3.3.4
heme
noncompetitive, reversible by dilution
Saccharomyces cerevisiae
1.3.3.4
hemin
noncompetitive, reversible by dilution
Saccharomyces cerevisiae
1.3.3.4
iodoacetamide
slight inhibition
Saccharomyces cerevisiae
1.3.3.4
iodoacetate
slight inhibition
Saccharomyces cerevisiae
1.3.3.4
additional information
inhibited by high salt concentrations
Saccharomyces cerevisiae
1.3.3.4
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.3.4
0.0048
-
protoporphyrinogen-IX
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.3.4
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.3.4
180000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.3.4
protoporphyrinogen-IX + 3 O2
Saccharomyces cerevisiae
penultimate step of heme and chlorophyll biosynthesis
protoporphyrin-IX + 3 H2O2
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.3.4
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.3.4
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.3.4
0.0026
-
purified enzyme
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
1.3.3.4
-20°C, in 50 mM Tris-HCl in presence of 20% glycerol and 1 mM EDTA, several months, no loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.3.4
mesoporphyrinogen-IX + 3 O2
20% of the rate of protoporphyrinogen-IX
390982
Saccharomyces cerevisiae
mesoporphyrin-IX + 3 H2O2
-
-
-
?
1.3.3.4
protoporphyrinogen-IX + 3 O2
-
390982
Saccharomyces cerevisiae
protoporphyrin-IX + 3 H2O2
-
390982
Saccharomyces cerevisiae
?
1.3.3.4
protoporphyrinogen-IX + 3 O2
penultimate step of heme and chlorophyll biosynthesis
390982
Saccharomyces cerevisiae
protoporphyrin-IX + 3 H2O2
-
390982
Saccharomyces cerevisiae
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.3.4
40
-
inactivated above 40°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.3.4
7.5
-
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.3.4
6.5
9
-
Saccharomyces cerevisiae
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.3.4
4
9.5
unstable at higher and lower pH
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.3.4
cysteine
-
Saccharomyces cerevisiae
1.3.3.4
dithiothreitol
-
Saccharomyces cerevisiae
1.3.3.4
glutathione
-
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
1.3.3.4
sensitive towards trypsin and chymotrypsin
Saccharomyces cerevisiae
1.3.3.4
stable on dialysis
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.3.4
arsenite
slight inhibition
Saccharomyces cerevisiae
1.3.3.4
Co2+
inhibitory
Saccharomyces cerevisiae
1.3.3.4
Cu2+
inhibitory
Saccharomyces cerevisiae
1.3.3.4
heme
noncompetitive, reversible by dilution
Saccharomyces cerevisiae
1.3.3.4
hemin
noncompetitive, reversible by dilution
Saccharomyces cerevisiae
1.3.3.4
iodoacetamide
slight inhibition
Saccharomyces cerevisiae
1.3.3.4
iodoacetate
slight inhibition
Saccharomyces cerevisiae
1.3.3.4
additional information
inhibited by high salt concentrations
Saccharomyces cerevisiae
1.3.3.4
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.3.4
0.0048
-
protoporphyrinogen-IX
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.3.4
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.3.4
180000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.3.4
protoporphyrinogen-IX + 3 O2
Saccharomyces cerevisiae
penultimate step of heme and chlorophyll biosynthesis
protoporphyrin-IX + 3 H2O2
-
Saccharomyces cerevisiae
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.3.4
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.3.4
0.0026
-
purified enzyme
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.3.4
-20°C, in 50 mM Tris-HCl in presence of 20% glycerol and 1 mM EDTA, several months, no loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.3.4
mesoporphyrinogen-IX + 3 O2
20% of the rate of protoporphyrinogen-IX
390982
Saccharomyces cerevisiae
mesoporphyrin-IX + 3 H2O2
-
-
-
?
1.3.3.4
protoporphyrinogen-IX + 3 O2
-
390982
Saccharomyces cerevisiae
protoporphyrin-IX + 3 H2O2
-
390982
Saccharomyces cerevisiae
?
1.3.3.4
protoporphyrinogen-IX + 3 O2
penultimate step of heme and chlorophyll biosynthesis
390982
Saccharomyces cerevisiae
protoporphyrin-IX + 3 H2O2
-
390982
Saccharomyces cerevisiae
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.3.4
40
-
inactivated above 40°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.3.4
7.5
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.3.4
6.5
9
-
Saccharomyces cerevisiae
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.3.4
4
9.5
unstable at higher and lower pH
Saccharomyces cerevisiae