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Literature summary extracted from

  • Husselstein, T.; Schaller, H.; Gachotte, D.; Benveniste, P.
    DELTA7-sterol-C5-desaturase: molecular characterization and functional expression of wild-type and mutant alleles (1999), Plant Mol. Biol., 39, 891-906.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.20 expression in Saccharomyces cerevisiae erg3 mutant Nicotiana tabacum
1.14.19.20 wild-type enzyme and ste1 mutant enzyme expressed in Sacharomyces cerevisiae erg3 mutant, wild-type enzyme overexpressed in Arabidopsis ste1 mutant Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.20 Arabidopsis thaliana
-
-
-
1.14.19.20 Nicotiana tabacum
-
-
-
1.14.19.20 Saccharomyces cerevisiae
-
-
-
1.14.19.20 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.20 (24Z)-5alpha-stigmast-7,24(241)-dien-3beta-ol + O2 + reduced acceptor
-
 Zea mays (24Z)-stigmasta-5,7,24(241)-trien-3-beta-ol + acceptor + H2O
-
 ?
1.14.19.20 5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5
-
 Saccharomyces cerevisiae ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5
-
 ?
1.14.19.20 5alpha-ergost-7,24(241)-dien-3beta-ol + O2 + ferrocytochrome b5
-
 Arabidopsis thaliana ergosta-5,7,24(241)-trien-3beta-ol + H2O2 + ferricytochrome b5
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.20 NAD(P)H
-
 Saccharomyces cerevisiae
1.14.19.20 NAD(P)H
-
 Zea mays
1.14.19.20 NAD(P)H
-
 Arabidopsis thaliana