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Literature summary extracted from

  • Bader, B.; Knecht, W.; Fries, M.; Loffler, M.
    Expression, purification, and characterization of histidine-tagged rat and human flavoenzyme dihydroorotate dehydrogenase (1998), Protein Expr. Purif., 13, 414-422.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.5.2 medicine
-
 Homo sapiens
1.3.5.2 medicine
-
 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.2 expressed in Escherichia coli XL-1 Blue, histidine tagged Homo sapiens
1.3.5.2 expressed in Escherichia coli XL-1 Blue, histidine tagged Rattus norvegicus
1.3.5.2 expression of complete catalytically active enzyme in SF21 insect cells of Spodoptera frugiperda with baculovirus vector system Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide inbibits by interference with the transfer of electrons from flavin to quinone Homo sapiens
1.3.5.2 A77 1726
-
 Homo sapiens
1.3.5.2 A77 1726
-
 Rattus norvegicus
1.3.5.2 brequinar sodium inbibits by interference with the transfer of electrons from flavin to quinone Homo sapiens
1.3.5.2 brequinar sodium
-
 Rattus norvegicus
1.3.5.2 leflunomide
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.0062
-
 S-dihydroorotate expressed in Escherichia coli Homo sapiens
1.3.5.2 0.0065
-
 decylubiquinone expressed in SF21 insect cells Rattus norvegicus
1.3.5.2 0.0095
-
 decyclubiquinone recombinant enzyme Rattus norvegicus
1.3.5.2 0.0095
-
 decylubiquinone expressed in Escherichia coli Rattus norvegicus
1.3.5.2 0.0135
-
 S-dihydroorotate expressed in SF21 insect cells Rattus norvegicus
1.3.5.2 0.0137
-
 decylubiquinone expressed in Escherichia coli Homo sapiens
1.3.5.2 0.0146
-
 S-dihydroorotate expressed in Escherichia coli Rattus norvegicus
1.3.5.2 0.015
-
 dihydroorotate recombinant enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion
-
 Homo sapiens 5739
-
1.3.5.2 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 45000
-
 x * 45100, SDS-PAGE, recombinant from SF21 insect cells, x * 45000, SDS-PAGE, recombinant from Escherichia coli Rattus norvegicus
1.3.5.2 45100
-
 x * 45100, SDS-PAGE, recombinant from SF21 insect cells, x * 45000, SDS-PAGE, recombinant from Escherichia coli Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone Homo sapiens six-step biosynthesis of pyrimidine uridine monophosphate orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone Rattus norvegicus six-step biosynthesis of pyrimidine uridine monophosphate orotate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Homo sapiens
-
-
-
1.3.5.2 Homo sapiens
-
 recombinant protein
-
1.3.5.2 Rattus norvegicus
-
-
-
1.3.5.2 Rattus norvegicus
-
 recombinant protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2 Ni2+/nitrilotriacetate column Homo sapiens
1.3.5.2 Ni2+/nitrilotriacetate column Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 83
-
 expressed in Escherichia coli Rattus norvegicus
1.3.5.2 130
-
 expressed in SF21 insect cells Rattus norvegicus
1.3.5.2 130 150
-
 Rattus norvegicus
1.3.5.2 150
-
-
 Homo sapiens
1.3.5.2 150
-
 expressed in Escherichia coli Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + decylubiquinone
-
 Rattus norvegicus orotate + decylubiquinol
-
 r
1.3.5.2 (S)-dihydroorotate + ubiquinone
-
 Rattus norvegicus orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone decylubiquinone as electron acceptor Homo sapiens orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone six-step biosynthesis of pyrimidine uridine monophosphate Homo sapiens orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone six-step biosynthesis of pyrimidine uridine monophosphate Rattus norvegicus orotate + ubiquinol
-
 ?
1.3.5.2 dihydroorotate + acceptor
-
 Homo sapiens orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor
-
 Rattus norvegicus orotate + reduced acceptor
-
 ?
1.3.5.2 L-dihydroorotate + decylubiquinone
-
 Homo sapiens orotate + reduced decylubiquinone
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 ?
-
 Homo sapiens
1.3.5.2 ? x * 45100, SDS-PAGE, recombinant from SF21 insect cells, x * 45000, SDS-PAGE, recombinant from Escherichia coli Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.2 8
-
-
 Homo sapiens
1.3.5.2 8
-
-
 Rattus norvegicus
1.3.5.2 8 8.1
-
 Homo sapiens
1.3.5.2 8 8.1
-
 Rattus norvegicus
1.3.5.2 8 8.1 recombinant enzymes with His-tags Homo sapiens
1.3.5.2 8 8.1 recombinant enzymes with His-tags Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 flavin
-
 Rattus norvegicus
1.3.5.2 FMN 0.5-0.8 mol/mol Homo sapiens
1.3.5.2 FMN 0.8-1.1 mol/mol Rattus norvegicus