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Literature summary extracted from

  • Taylor, W.H.; Taylor, C.D.; Taylor, M.L.
    Biosynthetic dihydroorotate dehydrogenase from Lactobacillus bulgaricus: partial characterization of the enzyme (1974), J. Bacteriol., 119, 98-105.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.98.1 2-mercaptoethanol 0.175 mM restores inhibited enzyme after 3 min incubation to 90% of full activity Lactobacillus delbrueckii subsp. bulgaricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.98.1 HgCl2
-
 Lactobacillus delbrueckii subsp. bulgaricus
1.3.98.1 Mercuric chloride 40% inhibition after 15 min at 0.000045 mM Lactobacillus delbrueckii subsp. bulgaricus
1.3.98.1 additional information markable sensitivity to sulfhydryl inhibitors Lactobacillus delbrueckii subsp. bulgaricus
1.3.98.1 N-ethylmaleimide
-
 Lactobacillus delbrueckii subsp. bulgaricus
1.3.98.1 Orotate competitive to dihydroorotate in ferricyanide reduction assay Lactobacillus delbrueckii subsp. bulgaricus
1.3.98.1 p-hydroxymercuribenzoate 98% inhibition after 5 min at 0.00227 mM Lactobacillus delbrueckii subsp. bulgaricus
1.3.98.1 Tiron inhibits cytochrome c reduction and sulfite autoxidation Lactobacillus delbrueckii subsp. bulgaricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.98.1 0.5
-
 S-dihydroorotate with ferricyanide as electron acceptor Lactobacillus delbrueckii subsp. bulgaricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.98.1 57000
-
 gel filtration Lactobacillus delbrueckii subsp. bulgaricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.98.1 (S)-dihydroorotate + O2 Lactobacillus delbrueckii subsp. bulgaricus pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme orotate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.1 Lactobacillus delbrueckii subsp. bulgaricus
-
 09, ATCC 13866
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1 preparative gel electrophoresis Lactobacillus delbrueckii subsp. bulgaricus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.98.1 (S)-dihydroorotate + fumarate = orotate + succinate sulfhydryl groups suggested to be located near the active site, suggested that enzyme produces superoxide free radicals upon reaction with oxygen Lactobacillus delbrueckii subsp. bulgaricus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.1 (S)-dihydroorotate + ferricyanide ferricyanide usually used as electon acceptor in the assay Lactobacillus delbrueckii subsp. bulgaricus orotate + ferrocyanide
-
 ?
1.3.98.1 (S)-dihydroorotate + O2 ferricyanide, cytochrome c and 2,6-dichlorophenolindolphenol as alternate electron acceptor Lactobacillus delbrueckii subsp. bulgaricus orotate + H2O2
-
 ?
1.3.98.1 (S)-dihydroorotate + O2 pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme Lactobacillus delbrueckii subsp. bulgaricus orotate + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.98.1 FMN flavoprotein, enzyme contains 1 FMN as prosthetic group Lactobacillus delbrueckii subsp. bulgaricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.98.1 0.1
-
 Orotate
-
 Lactobacillus delbrueckii subsp. bulgaricus