BRENDA - Enzyme Database

Fumarate reductase activity of Streptococcus faecalis

Aue, B.J.; Deibel, R.H.; J. Bacteriol. 93, 1770-1776 (1967)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.1.6
alanine
-
Enterococcus faecalis
1.3.1.6
glycine
-
Enterococcus faecalis
1.3.1.6
histidine
-
Enterococcus faecalis
General Stability
EC Number
General Stability
Organism
1.3.1.6
stabilized against air oxidation with 10 mM thioglycollate
Enterococcus faecalis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.1.6
citraconate
slight inhibition
Enterococcus faecalis
1.3.1.6
iodoacetate
slight inhibition
Enterococcus faecalis
1.3.1.6
malonate
slight inhibition
Enterococcus faecalis
1.3.1.6
p-chloromercuribenzoate
slight inhibition
Enterococcus faecalis
1.3.1.6
succinate
slight inhibition
Enterococcus faecalis
1.3.1.6
trans-, trans muconate
slight inhibition
Enterococcus faecalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.1.6
0.2
-
FMNH2
-
Enterococcus faecalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.6
fumarate + NADH + H+
Enterococcus faecalis
-
succinate + NAD+
-
Enterococcus faecalis
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.6
Enterococcus faecalis
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.3.1.6
sensitive to air oxidation, loss of activity
Enterococcus faecalis
Purification (Commentary)
EC Number
Commentary
Organism
1.3.1.6
-
Enterococcus faecalis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.6
0.23
-
in presence of 10 mM thioglycolate
Enterococcus faecalis
Storage Stability
EC Number
Storage Stability
Organism
1.3.1.6
stable in lyophylized whole cells
Enterococcus faecalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.6
fumarate + NADH + H+
-
390836
Enterococcus faecalis
succinate + NAD+
-
390836
Enterococcus faecalis
-
1.3.1.6
fumarate + NADH + H+
FMNH2 serves as cofactor
390836
Enterococcus faecalis
succinate + NAD+
-
390836
Enterococcus faecalis
ir
1.3.1.6
maleate + NADH
-
390836
Enterococcus faecalis
succinate + NAD+
-
-
-
ir
1.3.1.6
mesaconate + NADH
-
390836
Enterococcus faecalis
methylsuccinate + NAD+
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.1.6
7.4
-
-
Enterococcus faecalis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.1.6
5
8
-
Enterococcus faecalis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.6
FMNH2
-
Enterococcus faecalis
1.3.1.6
NADH
-
Enterococcus faecalis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.1.6
alanine
-
Enterococcus faecalis
1.3.1.6
glycine
-
Enterococcus faecalis
1.3.1.6
histidine
-
Enterococcus faecalis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.6
FMNH2
-
Enterococcus faecalis
1.3.1.6
NADH
-
Enterococcus faecalis
General Stability (protein specific)
EC Number
General Stability
Organism
1.3.1.6
stabilized against air oxidation with 10 mM thioglycollate
Enterococcus faecalis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.1.6
citraconate
slight inhibition
Enterococcus faecalis
1.3.1.6
iodoacetate
slight inhibition
Enterococcus faecalis
1.3.1.6
malonate
slight inhibition
Enterococcus faecalis
1.3.1.6
p-chloromercuribenzoate
slight inhibition
Enterococcus faecalis
1.3.1.6
succinate
slight inhibition
Enterococcus faecalis
1.3.1.6
trans-, trans muconate
slight inhibition
Enterococcus faecalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.1.6
0.2
-
FMNH2
-
Enterococcus faecalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.6
fumarate + NADH + H+
Enterococcus faecalis
-
succinate + NAD+
-
Enterococcus faecalis
-
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.3.1.6
sensitive to air oxidation, loss of activity
Enterococcus faecalis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.6
-
Enterococcus faecalis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.6
0.23
-
in presence of 10 mM thioglycolate
Enterococcus faecalis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.1.6
stable in lyophylized whole cells
Enterococcus faecalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.6
fumarate + NADH + H+
-
390836
Enterococcus faecalis
succinate + NAD+
-
390836
Enterococcus faecalis
-
1.3.1.6
fumarate + NADH + H+
FMNH2 serves as cofactor
390836
Enterococcus faecalis
succinate + NAD+
-
390836
Enterococcus faecalis
ir
1.3.1.6
maleate + NADH
-
390836
Enterococcus faecalis
succinate + NAD+
-
-
-
ir
1.3.1.6
mesaconate + NADH
-
390836
Enterococcus faecalis
methylsuccinate + NAD+
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.1.6
7.4
-
-
Enterococcus faecalis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.1.6
5
8
-
Enterococcus faecalis