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Literature summary extracted from

  • Ensor, C.M.; Zhang, H.; Tai, H.H.
    Purification, cDNA cloning and expression of 15-oxoprostaglandin 13-reductase from pig lung (1998), Biochem. J., 330, 103-108.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.48 expression in Escherichia coli Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.48 0.0077
-
 15-ketoprostaglandin E1
-
 Sus scrofa
1.3.1.48 0.0083
-
 15-ketoprostaglandin E1 recombinant enzyme Sus scrofa
1.3.1.48 0.01
-
 leukotriene B4 recombinant enzyme Sus scrofa
1.3.1.48 0.015
-
 NADPH
-
 Sus scrofa
1.3.1.48 0.016
-
 NADPH recombinant enzyme Sus scrofa
1.3.1.48 0.0182
-
 15-ketoprostaglandin E2 recombinant enzyme Sus scrofa
1.3.1.48 0.019
-
 15-ketoprostaglandin E2
-
 Sus scrofa
1.3.1.48 0.088 0.094 NADH
-
 Bos taurus
1.3.1.48 0.153
-
 NADH
-
 Sus scrofa
1.3.1.48 0.16
-
 NADH recombinant enzyme Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.48 Bos taurus
-
-
-
1.3.1.48 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.48 native and recombinant enzyme Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.48 lung
-
 Sus scrofa
-
1.3.1.48 lung
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.48 2.56
-
-
 Sus scrofa
1.3.1.48 2.6
-
 recombinant enzyme expressed in Escherichia coli Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.48 (5S,12R)-dihydroxy-(6E,8Z,10Z,14E)-eicosatetraenoic acid + NADH trivial name leukotriene B4, enzyme exhibits a 15-ketoprostaglandin-13-reductase and a very low leukotriene B4 12-hydroxydehydrogenase activity Sus scrofa ?
-
 ?
1.3.1.48 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
 Sus scrofa (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
 ir
1.3.1.48 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
 Bos taurus (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
 ir
1.3.1.48 11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
 Sus scrofa 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
 ?
1.3.1.48 11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
 Bos taurus 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
 ?
1.3.1.48 15-ketoprostaglandin E1 + NADPH
-
 Sus scrofa ?
-
 ?
1.3.1.48 15-ketoprostaglandin E2 + NADH + H+
-
 Bos taurus 13,14-dihydro-15-ketoprostaglandin E2 + NAD+
-
 r
1.3.1.48 leukotriene B4 + NADPH
-
 Sus scrofa ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.48 0.517
-
 15-ketoprostaglandin E1 cofactor NADH Sus scrofa
1.3.1.48 1.52
-
 15-ketoprostaglandin E1 cofactor NADPH Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.48 NAD(P)H
-
 Bos taurus
1.3.1.48 NAD(P)H 3fold higher activity with NADPH compared to NADH Sus scrofa