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Literature summary extracted from

  • Schaller, F.; Biesgen, C.; Muessig, C.; Altmann, T.; Weiler, E.W.
    12-Oxophytodienoate reductase 3 (OPR3) is the isoenzyme involved in jasmonate biosynthesis (2000), Planta, 210, 979-984.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.42 OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.42 0.035
-
 (9S,13S)-12-oxophytodienoic acid
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.42 (9S,13S)-12-oxo-phytodienoic acid + NADPH Arabidopsis thaliana OPR3 is the isoenzyme relevant for jasmonate biosynthesis 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
 ?
1.3.1.42 (9S,13S)-12-oxo-phytodienoic acid + NADPH Capnoides sempervirens reductase specific for the natural cis-OPDA enatiomer encoded by OPR2 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.42 Arabidopsis thaliana
-
 (L.) Heynta wild-type ecotype Columbia(Col-0)
-
1.3.1.42 Capnoides sempervirens
-
-
-
1.3.1.42 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.42
-
 Saccharomyces cerevisiae
1.3.1.42
-
 Capnoides sempervirens
1.3.1.42 OPR1, OPR2 and OPR3 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.42 (9S,13S)-12-oxo-phytodienoic acid + NADPH OPR3 is the isoenzyme relevant for jasmonate biosynthesis Arabidopsis thaliana 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
 ?
1.3.1.42 (9S,13S)-12-oxo-phytodienoic acid + NADPH reductase specific for the natural cis-OPDA enatiomer encoded by OPR2 Capnoides sempervirens 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.42 OPR1
-
 Arabidopsis thaliana
1.3.1.42 OPR1
-
 Capnoides sempervirens
1.3.1.42 OPR2
-
 Arabidopsis thaliana
1.3.1.42 OPR3
-
 Arabidopsis thaliana
1.3.1.42 OYE
-
 Saccharomyces cerevisiae