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Literature summary extracted from

  • Mueller, D.; Schloemann, M.; Reineke, W.
    Maleylacetate reductases in chloroaromatic-degrading bacteria using the modified ortho pathway: comparison of catalytic properties (1996), J. Bacteriol., 178, 298-300.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.32 0.014
-
 5-fluoromaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.022
-
 5-fluoromaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.029
-
 3-chloromaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.03
-
 3-chloromaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.044
-
 5-chloromaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.05
-
 maleylacetate
-
 Cupriavidus necator
1.3.1.32 0.05
-
 5-chloromaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.052
-
 2-chloromaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.063
-
 3-bromomaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.065
-
 maleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.071
-
 3-bromomaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.078
-
 2-chloromaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.084
-
 5-bromomaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.095
-
 5-bromomaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.098
-
 2-bromomaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.117
-
 2-bromomaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.125
-
 5-methylmaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.133
-
 5-methylmaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.17
-
 2,3-dichloromaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.288
-
 2,5-dichloromaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.292
-
 3-methylmaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.33
-
 2-methylmaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.365
-
 3-methylmaleylacetate
-
 Pseudomonas aeruginosa
1.3.1.32 0.374
-
 2-methylmaleylacetate
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 37000
-
 2 * 37000, SDS-PAGE Pseudomonas aeruginosa
1.3.1.32 74000
-
-
 Pseudomonas aeruginosa
1.3.1.32 74000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH Pseudomonas aeruginosa
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Pseudomonas sp.
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cupriavidus necator
-
 3-oxoadipate + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Cupriavidus necator
-
 JMP 134
-
1.3.1.32 Cupriavidus necator JMP 134-1
-
 JMP 134
-
1.3.1.32 Pseudomonas aeruginosa
-
-
-
1.3.1.32 Pseudomonas aeruginosa RHO1
-
-
-
1.3.1.32 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32
-
 Pseudomonas aeruginosa
1.3.1.32
-
 Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.32 170
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2,3,5-trichloromaleylacetate + NADH
-
 Pseudomonas aeruginosa 2,4-dichloro-3-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,3,5-trichloromaleylacetate + NADH
-
 Pseudomonas sp. 2,4-dichloro-3-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,3,5-trichloromaleylacetate + NADH
-
 Cupriavidus necator 2,4-dichloro-3-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,3-dibromomaleylacetate + NADH
-
 Pseudomonas aeruginosa 3-bromo-4-oxohexanedioic acid + NAD+ + Br-
-
 ?
1.3.1.32 2,3-dibromomaleylacetate + NADH
-
 Pseudomonas sp. 3-bromo-4-oxohexanedioic acid + NAD+ + Br-
-
 ?
1.3.1.32 2,3-dibromomaleylacetate + NADH
-
 Cupriavidus necator 3-bromo-4-oxohexanedioic acid + NAD+ + Br-
-
 ?
1.3.1.32 2,3-dichloromaleylacetate + NADH
-
 Pseudomonas aeruginosa 3-chloro-4-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,3-dichloromaleylacetate + NADH
-
 Pseudomonas sp. 3-chloro-4-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,3-dichloromaleylacetate + NADH
-
 Cupriavidus necator 3-chloro-4-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,5-dichloromaleylacetate + NADH
-
 Pseudomonas aeruginosa 2-chloro-3-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,5-dichloromaleylacetate + NADH
-
 Pseudomonas sp. 2-chloro-3-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2,5-dichloromaleylacetate + NADH
-
 Cupriavidus necator 2-chloro-3-oxohexanedioic acid + NAD+ + Cl-
-
 ?
1.3.1.32 2-bromomaleylacetate + NADH
-
 Pseudomonas aeruginosa 2-maleylacetate + NAD+ + Br-
-
 ?
1.3.1.32 2-bromomaleylacetate + NADH
-
 Cupriavidus necator 2-maleylacetate + NAD+ + Br-
-
 ?
1.3.1.32 2-bromomaleylacetate + NADH
-
 Pseudomonas sp. 2-maleylacetate + NAD+ + Br- the product is further reduced to 2-bromo-3-oxohexanedioic acid under consumption of NADH ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas aeruginosa 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas sp. 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Cupriavidus necator 2-maleylacetate + Cl- + NAD+ the product is further reduced to 3-oxoadipate under consumption of NADH ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas aeruginosa RHO1 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Cupriavidus necator JMP 134-1 2-maleylacetate + Cl- + NAD+ the product is further reduced to 3-oxoadipate under consumption of NADH ?
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cupriavidus necator 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas aeruginosa 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas sp. 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cupriavidus necator 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-bromomaleylacetate + NADH
-
 Pseudomonas aeruginosa 3-bromo-4-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 3-bromomaleylacetate + NADH
-
 Pseudomonas sp. 3-bromo-4-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 3-bromomaleylacetate + NADH
-
 Cupriavidus necator 3-bromo-4-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 3-chloromaleylacetate + NADH
-
 Pseudomonas aeruginosa 3-chloro-4-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 3-chloromaleylacetate + NADH
-
 Pseudomonas sp. 3-chloro-4-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 3-chloromaleylacetate + NADH
-
 Cupriavidus necator 3-chloro-4-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 5-bromomaleylacetate + NADH
-
 Pseudomonas aeruginosa 2-bromo-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-bromomaleylacetate + NADH
-
 Pseudomonas sp. 2-bromo-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-bromomaleylacetate + NADH
-
 Cupriavidus necator 2-bromo-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-chloromaleylacetate + NADH
-
 Pseudomonas aeruginosa 2-chloro-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-chloromaleylacetate + NADH
-
 Pseudomonas sp. 2-chloro-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-chloromaleylacetate + NADH
-
 Cupriavidus necator 2-chloro-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-fluoromaleylacetate + NADH
-
 Pseudomonas aeruginosa 2-fluoro-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-fluoromaleylacetate + NADH
-
 Cupriavidus necator 2-fluoro-3-oxohexanedioic acid + NAD+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 2-methyl-3-oxoadipate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.32 dimer
-
 Pseudomonas sp.
1.3.1.32 dimer
-
 Cupriavidus necator
1.3.1.32 dimer 2 * 37000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 MAR
-
 Pseudomonas aeruginosa
1.3.1.32 MAR
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.32 45
-
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 5.8
-
-
 Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.32 5.8
-
 activity drastically reduced at pH values below Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Pseudomonas sp.
1.3.1.32 NADH
-
 Cupriavidus necator