Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cheng, Y.J.; Karavolas, H.J.
    Subcellular distribution and properties of progesterone (delta4-steroid) 5alpha-reductase in rat medial basal hypothalamus (1975), J. Biol. Chem., 250, 7997-8003.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.22 17beta-estradiol noncompetitive inhibition Rattus norvegicus
1.3.1.22 20alpha-hydroxypregn-4-en-3-one competitive Rattus norvegicus
1.3.1.22 testosterone poor competitive inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.22 additional information
-
 additional information
-
 Rattus norvegicus
1.3.1.22 0.00048
-
 progesterone
-
 Rattus norvegicus
1.3.1.22 0.00086
-
 20alpha-hydroxypregn-4-en-3-one
-
 Rattus norvegicus
1.3.1.22 0.016
-
 testosterone
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.22 Rattus norvegicus
-
 female rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.22 hypothalamus
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.22 20alpha-hydroxy-4-pregnen-3-one + NADPH more reactive than progesterone Rattus norvegicus 20alpha-hydroxy-5-beta-pregnan-3-one + NADP+
-
 ?
1.3.1.22 additional information testosterone is less effective Rattus norvegicus ?
-
 ?
1.3.1.22 progesterone + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-3-20-dione + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.22 32
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.22 15 42 15°C: about 50% of activity maximum, 42°C: about 30% of activity maximum Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.22 6 7.2
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.22 5 8 pH 5.0: about 20% of activity maximum, pH 8.0: about 20% of activity maximum Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.22 NADPH cannot be replaced by NADH Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.22 0.00006
-
 20alpha-hydroxypregn-4-en-3-one
-
 Rattus norvegicus
1.3.1.22 0.026
-
 17beta-estradiol intercept inhibition constant Rattus norvegicus
1.3.1.22 0.036
-
 17beta-estradiol slope inhibition constant Rattus norvegicus