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Literature summary extracted from

  • Van Doorn, E.J.; Nduaguba, J.C.; Clark, A.F.
    Studies on partially purified pig liver steroid DELTA4-5beta-reductase activity (1973), Can. J. Biochem., 51, 1661-1668.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.3 5beta-dihydrotestosterone product inhibition Sus scrofa
1.3.1.3 iodoacetate
-
 Sus scrofa
1.3.1.3 N-ethylmaleimide
-
 Sus scrofa
1.3.1.3 NADP+ product inhibition Sus scrofa
1.3.1.3 p-chloromercuribenzenesulfonate
-
 Sus scrofa
1.3.1.3 p-chloromercuribenzoate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.085
-
 NADPH
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.3 cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.3 25000 30000 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 liver
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
1.3.1.3 4°C, 72 h, 0.05 M Tris-maleate buffer, pH 7.4-8.4, stable Sus scrofa
1.3.1.3 4°C, pH 8, 32 days, 25% loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 aldosterone + NADPH
-
 Sus scrofa ?
-
 ?
1.3.1.3 additional information enzyme reduces a variety of C19 and C21 steroids, highest activity with aldosterone Sus scrofa ?
-
 ?
1.3.1.3 testosterone + NADPH + H+
-
 Sus scrofa (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.3 6.4
-
 Tris-maleate buffer Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.3 5.7 8 pH 5.7: about 50% of activity maximum, pH 8.0: about 70% of activity maximum Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.3 8
-
 4°C, 30 days, 25% loss of activity Sus scrofa