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Literature summary extracted from

  • Okuda, A.; Okuda, K.
    Purification and characterization of DELTA 4-3-ketosteroid 5beta-reductase (1984), J. Biol. Chem., 259, 7519-7524.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.3 highly labile in low buffer concentration Rattus norvegicus
1.3.1.3 markedly stabilized in 20% glycerol Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.3 p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.00219
-
 progesterone
-
 Rattus norvegicus
1.3.1.3 0.0024
-
 testosterone
-
 Rattus norvegicus
1.3.1.3 0.003
-
 NADPH
-
 Rattus norvegicus
1.3.1.3 0.00485
-
 4-androstene-3,17-dione
-
 Rattus norvegicus
1.3.1.3 0.0091
-
 7alpha-hydroxy-4-cholesten-3-one
-
 Rattus norvegicus
1.3.1.3 0.011
-
 cortisol
-
 Rattus norvegicus
1.3.1.3 0.0122
-
 7alpha,12alpha-dihydroxy-4-cholesten-3-one
-
 Rattus norvegicus
1.3.1.3 0.0217
-
 cortisone
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.3 37000
-
 SDS-PAGE, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.3 4-androstene-3,17-dione + NADPH Rattus norvegicus
-
 5beta-androstan-3,17-dione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3 cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.3 0.01528
-
-
 Rattus norvegicus
1.3.1.3 0.0153
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 4-androstene-3,17-dione + NADPH
-
 Rattus norvegicus 5beta-androstan-3,17-dione + NADP+
-
 ?
1.3.1.3 7alpha,12alpha-dihydroxy-4-cholesten-3-one + NADPH
-
 Rattus norvegicus 7alpha,12alpha-dihydroxy-5-beta-cholestan-3-one + NADP+
-
 ?
1.3.1.3 7alpha-hydroxy-4-cholesten + NADPH
-
 Rattus norvegicus 7alpha-hydroxy-5beta-cholestan-3-one + NADP+
-
 ?
1.3.1.3 7alpha-hydroxy-4-cholesten-3-one + NADPH + H+
-
 Rattus norvegicus 7alpha-hydroxy-5beta-cholestan-3-one + NADP+
-
 r
1.3.1.3 cortisol + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.3 cortisone + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.3 additional information NADH: no effect Rattus norvegicus ?
-
 ?
1.3.1.3 progesterone + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.3 testosterone + NADPH + H+
-
 Rattus norvegicus (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.3 0.00817
-
 cortisol
-
 Rattus norvegicus
1.3.1.3 0.01
-
 7alpha-hydroxy-4-cholesten-3-one
-
 Rattus norvegicus
1.3.1.3 0.0128
-
 7alpha,12alpha-dihydroxy-4-cholesten-3-one
-
 Rattus norvegicus
1.3.1.3 0.0208
-
 testosterone
-
 Rattus norvegicus
1.3.1.3 0.0302
-
 progesterone
-
 Rattus norvegicus
1.3.1.3 0.0337
-
 4-androstene-3,17-dione
-
 Rattus norvegicus
1.3.1.3 0.0375
-
 cortisone
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.3 7.4
-
 7alpha-hydroxy-4-cholesten-3-one Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.3 5.8 8.2
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 additional information NADH: no effect Rattus norvegicus
1.3.1.3 NADPH
-
 Rattus norvegicus