BRENDA - Enzyme Database

Studies on the metabolism of kynurenic acid

Taniuchi, H.; Hayaishi, O.; J. Biol. Chem. 238, 283-293 (1963)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.14.99.2
2-mercaptoethanol stabilizes activity
Pseudomonas fluorescens
1.14.99.2
cysteine stabilizes activity
Pseudomonas fluorescens
1.14.99.2
GSH stabilizes activity
Pseudomonas fluorescens
1.14.99.2
precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activity
Pseudomonas fluorescens
1.14.99.2
thioglycolate stabilizes activity
Pseudomonas fluorescens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.99.2
Ag+
-
Pseudomonas fluorescens
1.14.99.2
Cu2+
-
Pseudomonas fluorescens
1.14.99.2
Hg2+
-
Pseudomonas fluorescens
1.14.99.2
iodoacetate
inhibition reversed by addition of GSH or cysteine
Pseudomonas fluorescens
1.14.99.2
Kynurenate
competitive inhibition at 0.2 mM
Pseudomonas fluorescens
1.14.99.2
p-chloromercuribenzoate
inhibition reversed by addition of GSH or cysteine
Pseudomonas fluorescens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.99.2
0.008
-
Kynurenate
-
Pseudomonas fluorescens
1.14.99.2
0.04
-
NADH
-
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.99.2
kynurenate + NADH + O2
Pseudomonas fluorescens
-
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
-
-
?
1.14.99.2
kynurenate + NADPH + O2
Pseudomonas fluorescens
-
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.18
Pseudomonas fluorescens
-
ATCC 11299b
-
1.14.99.2
Pseudomonas fluorescens
-
ATCC 11299b
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.1.18
partial
Pseudomonas fluorescens
1.14.99.2
partial
Pseudomonas fluorescens
Storage Stability
EC Number
Storage Stability
Organism
1.14.99.2
-10C, ammonium sulfate fractions, loss of activity after a few days
Pseudomonas fluorescens
1.14.99.2
-10C, supernatant after high speed centrifugation of crude extract, several months, no loss in activity
Pseudomonas fluorescens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.18
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
-
390584
Pseudomonas fluorescens
7,8-dihydroxykynurenate + NADH
-
390584
Pseudomonas fluorescens
ir
1.14.99.2
kynurenate + NADH + O2
-
390584
Pseudomonas fluorescens
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
-
-
-
?
1.14.99.2
kynurenate + NADPH + O2
-
390584
Pseudomonas fluorescens
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.3.1.18
23
-
assay at
Pseudomonas fluorescens
1.14.99.2
23
-
assay at
Pseudomonas fluorescens
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.14.99.2
60
-
5 min, complete inactivation
Pseudomonas fluorescens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.2
7
-
-
Pseudomonas fluorescens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.99.2
9
-
stable in presence 10 mM cysteine
Pseudomonas fluorescens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.18
NAD+
-
Pseudomonas fluorescens
1.14.99.2
NADH
-
Pseudomonas fluorescens
1.14.99.2
NADPH
-
Pseudomonas fluorescens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.18
NAD+
-
Pseudomonas fluorescens
1.14.99.2
NADH
-
Pseudomonas fluorescens
1.14.99.2
NADPH
-
Pseudomonas fluorescens
General Stability (protein specific)
EC Number
General Stability
Organism
1.14.99.2
2-mercaptoethanol stabilizes activity
Pseudomonas fluorescens
1.14.99.2
cysteine stabilizes activity
Pseudomonas fluorescens
1.14.99.2
GSH stabilizes activity
Pseudomonas fluorescens
1.14.99.2
precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activity
Pseudomonas fluorescens
1.14.99.2
thioglycolate stabilizes activity
Pseudomonas fluorescens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.99.2
Ag+
-
Pseudomonas fluorescens
1.14.99.2
Cu2+
-
Pseudomonas fluorescens
1.14.99.2
Hg2+
-
Pseudomonas fluorescens
1.14.99.2
iodoacetate
inhibition reversed by addition of GSH or cysteine
Pseudomonas fluorescens
1.14.99.2
Kynurenate
competitive inhibition at 0.2 mM
Pseudomonas fluorescens
1.14.99.2
p-chloromercuribenzoate
inhibition reversed by addition of GSH or cysteine
Pseudomonas fluorescens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.99.2
0.008
-
Kynurenate
-
Pseudomonas fluorescens
1.14.99.2
0.04
-
NADH
-
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.99.2
kynurenate + NADH + O2
Pseudomonas fluorescens
-
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
-
-
?
1.14.99.2
kynurenate + NADPH + O2
Pseudomonas fluorescens
-
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.18
partial
Pseudomonas fluorescens
1.14.99.2
partial
Pseudomonas fluorescens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.99.2
-10C, ammonium sulfate fractions, loss of activity after a few days
Pseudomonas fluorescens
1.14.99.2
-10C, supernatant after high speed centrifugation of crude extract, several months, no loss in activity
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.18
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
-
390584
Pseudomonas fluorescens
7,8-dihydroxykynurenate + NADH
-
390584
Pseudomonas fluorescens
ir
1.14.99.2
kynurenate + NADH + O2
-
390584
Pseudomonas fluorescens
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
-
-
-
?
1.14.99.2
kynurenate + NADPH + O2
-
390584
Pseudomonas fluorescens
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.3.1.18
23
-
assay at
Pseudomonas fluorescens
1.14.99.2
23
-
assay at
Pseudomonas fluorescens
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.14.99.2
60
-
5 min, complete inactivation
Pseudomonas fluorescens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.2
7
-
-
Pseudomonas fluorescens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.99.2
9
-
stable in presence 10 mM cysteine
Pseudomonas fluorescens