EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.14 | cysteine | essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not | Faecalicatena orotica | |
1.3.1.14 | glutathione | stimulating effect, but less effective than cysteine | Faecalicatena orotica | |
1.3.1.14 | thioglycolate | stimulating effect, but less effective than cysteine | Faecalicatena orotica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.14 | 1,10-phenanthroline | 1 mM, 10% inhibition | Faecalicatena orotica | |
1.3.1.14 | 2,4-Dihydroxy-6-methyl pyrimidine | - |
Faecalicatena orotica | |
1.3.1.14 | Acriflavin | partial inhibition | Faecalicatena orotica | |
1.3.1.14 | Barbituric acid | - |
Faecalicatena orotica | |
1.3.1.14 | p-chloromercuribenzoate | completely inhibited by 0.1 mM | Faecalicatena orotica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.14 | 0.13 | - |
Orotate | - |
Faecalicatena orotica | |
1.3.1.14 | 0.13 | - |
5-Fluoroorotate | - |
Faecalicatena orotica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.14 | dihydroorotate + NAD+ | Faecalicatena orotica | enzyme presumably functions mainly in the metabolic synthesis of pyrimidines | orotate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.14 | Faecalicatena orotica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.14 | about 90fold purification | Faecalicatena orotica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.14 | additional information | - |
- |
Faecalicatena orotica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.14 | (S)-dihydroorotate + NAD+ | equilibrium favours direction of orotate reduction | Faecalicatena orotica | orotate + NADH + H+ | - |
r | |
1.3.1.14 | (S)-dihydroorotate + NAD+ | substrate is oxidized by NAD+ and oxygen | Faecalicatena orotica | orotate + NADH + H+ | - |
r | |
1.3.1.14 | 5-fluorodihydroorotate + NAD+ | more active substrate for reverse reaction than orotate | Faecalicatena orotica | 5-fluoroorotate + NADH + H+ | - |
r | |
1.3.1.14 | dihydroorotate + NAD+ | enzyme presumably functions mainly in the metabolic synthesis of pyrimidines | Faecalicatena orotica | orotate + NADH + H+ | - |
r | |
1.3.1.14 | additional information | 3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c | Faecalicatena orotica | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.14 | additional information | - |
additional information | values not per enzyme molecule, but per mol of flavin | Faecalicatena orotica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.14 | FAD | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica | |
1.3.1.14 | flavin | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica | |
1.3.1.14 | flavin | interaction of flavin and dihydroorotate is completely dependent on cysteine | Faecalicatena orotica | |
1.3.1.14 | FMN | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica |