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Literature summary extracted from

  • Friedmann, H.C.; Vennesland, B.
    Purification and properties of diihydroorotic dehydrogenase (1958), J. Biol. Chem., 233, 1398-1406.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.14 cysteine essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not Faecalicatena orotica
1.3.1.14 glutathione stimulating effect, but less effective than cysteine Faecalicatena orotica
1.3.1.14 thioglycolate stimulating effect, but less effective than cysteine Faecalicatena orotica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.14 1,10-phenanthroline 1 mM, 10% inhibition Faecalicatena orotica
1.3.1.14 2,4-Dihydroxy-6-methyl pyrimidine
-
 Faecalicatena orotica
1.3.1.14 Acriflavin partial inhibition Faecalicatena orotica
1.3.1.14 Barbituric acid
-
 Faecalicatena orotica
1.3.1.14 p-chloromercuribenzoate completely inhibited by 0.1 mM Faecalicatena orotica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.14 0.13
-
 Orotate
-
 Faecalicatena orotica
1.3.1.14 0.13
-
 5-Fluoroorotate
-
 Faecalicatena orotica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.14 dihydroorotate + NAD+ Faecalicatena orotica enzyme presumably functions mainly in the metabolic synthesis of pyrimidines orotate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.14 Faecalicatena orotica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.14 about 90fold purification Faecalicatena orotica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.14 additional information
-
-
 Faecalicatena orotica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.14 (S)-dihydroorotate + NAD+ equilibrium favours direction of orotate reduction Faecalicatena orotica orotate + NADH + H+
-
 r
1.3.1.14 (S)-dihydroorotate + NAD+ substrate is oxidized by NAD+ and oxygen Faecalicatena orotica orotate + NADH + H+
-
 r
1.3.1.14 5-fluorodihydroorotate + NAD+ more active substrate for reverse reaction than orotate Faecalicatena orotica 5-fluoroorotate + NADH + H+
-
 r
1.3.1.14 dihydroorotate + NAD+ enzyme presumably functions mainly in the metabolic synthesis of pyrimidines Faecalicatena orotica orotate + NADH + H+
-
 r
1.3.1.14 additional information 3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c Faecalicatena orotica ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.14 additional information
-
 additional information values not per enzyme molecule, but per mol of flavin Faecalicatena orotica

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.14 FAD flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
1.3.1.14 flavin flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
1.3.1.14 flavin interaction of flavin and dihydroorotate is completely dependent on cysteine Faecalicatena orotica
1.3.1.14 FMN flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica