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Literature summary extracted from

  • Ahmad, S.; Jensen, R.A.
    Evolution of the biochemical pathway for aromatic amino acid biosynthesis in Serpens flexibilis in relationship to its phylogenetic position (1987), Arch. Microbiol., 147, 8-12.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.12 4-hydroxymercuribenzoate
-
 Pseudomonas flexibilis
1.3.1.12 L-tyrosine
-
 Pseudomonas flexibilis
1.3.1.43 L-tyrosine competitive inhibition Pseudomonas flexibilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.12 0.045 0.09 prephenate
-
 Pseudomonas flexibilis
1.3.1.12 0.45
-
 NAD+
-
 Pseudomonas flexibilis
1.3.1.43 0.41
-
 NAD+
-
 Pseudomonas flexibilis
1.3.1.43 0.66
-
 L-arogenate
-
 Pseudomonas flexibilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.12 prephenate + NAD+ Pseudomonas flexibilis biosynthesis of L-tyrosine 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
1.3.1.43 L-arogenate + NAD+ Pseudomonas flexibilis
-
 L-tyrosine + NADH + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.12 Pseudomonas flexibilis
-
-
-
1.3.1.43 Pseudomonas flexibilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.12 copurification with arogenate dehydrogenase Pseudomonas flexibilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.12 prephenate + NAD+
-
 Pseudomonas flexibilis 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
1.3.1.12 prephenate + NAD+ biosynthesis of L-tyrosine Pseudomonas flexibilis 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
1.3.1.43 L-arogenate + NAD(P)+
-
 Pseudomonas flexibilis L-tyrosine + NAD(P)H + CO2
-
 ?
1.3.1.43 L-arogenate + NAD+
-
 Pseudomonas flexibilis L-tyrosine + NADH + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.43 37
-
 assay at Pseudomonas flexibilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.12 additional information NADP+ cannot substitute for NAD+ Pseudomonas flexibilis
1.3.1.12 NAD+
-
 Pseudomonas flexibilis
1.3.1.43 NAD+
-
 Pseudomonas flexibilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.12 0.017
-
 L-tyrosine with prephenate as variable substrate Pseudomonas flexibilis
1.3.1.12 0.02
-
 L-tyrosine with NAD+ as variable substrate Pseudomonas flexibilis
1.3.1.43 0.016
-
 L-tyrosine competitive inhibition Pseudomonas flexibilis