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Literature summary extracted from

  • Kato, N.; Kobayashi, H.; Shimano, M.; Sakazawa, C.
    Properties of formaldehyde dismutation catalyzing enzyme of Pseudomonas putida F61 (1984), Agric. Biol. Chem., 48, 2017-2023.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.98.1 Hg2+
-
 Pseudomonas putida
1.2.98.1 hydroxylamine
-
 Pseudomonas putida
1.2.98.1 NaBH4
-
 Pseudomonas putida
1.2.98.1 Semicarbazide
-
 Pseudomonas putida
1.2.98.1 thiosulfate
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.98.1 55000
-
 4 * 55000, SDS-PAGE Pseudomonas putida
1.2.98.1 220000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.98.1 Pseudomonas putida
-
 2 forms
-
1.2.98.1 Pseudomonas putida
-
 F61
-
1.2.98.1 Pseudomonas putida F61
-
 F61
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.98.1
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.98.1 91.1
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.98.1 formaldehyde + H2O dismutation Pseudomonas putida formate + methanol
-
 ?
1.2.98.1 formaldehyde + H2O dismutation Pseudomonas putida F61 formate + methanol
-
 ?
1.2.98.1 formaldehyde + H2O + acrolein coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + allyl alcohol
-
 ?
1.2.98.1 formaldehyde + H2O + acrolein cross-dismutation Pseudomonas putida formate + allyl alcohol
-
 ?
1.2.98.1 formaldehyde + H2O + acrolein in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + allyl alcohol
-
 ?
1.2.98.1 formaldehyde + H2O + acrolein coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida F61 formate + allyl alcohol
-
 ?
1.2.98.1 formaldehyde + H2O + butyraldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + butanol
-
 ?
1.2.98.1 formaldehyde + H2O + butyraldehyde cross-dismutation Pseudomonas putida formate + butanol
-
 ?
1.2.98.1 formaldehyde + H2O + butyraldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + butanol
-
 ?
1.2.98.1 formaldehyde + H2O + crotonaldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + crotonyl alcohol
-
 ?
1.2.98.1 formaldehyde + H2O + crotonaldehyde cross-dismutation Pseudomonas putida formate + crotonyl alcohol
-
 ?
1.2.98.1 formaldehyde + H2O + crotonaldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + crotonyl alcohol
-
 ?
1.2.98.1 formaldehyde + H2O + isobutyraldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + isobutanol
-
 ?
1.2.98.1 formaldehyde + H2O + isobutyraldehyde cross-dismutation Pseudomonas putida formate + isobutanol
-
 ?
1.2.98.1 formaldehyde + H2O + isobutyraldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + isobutanol
-
 ?
1.2.98.1 formaldehyde + H2O + propionaldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida formate + propanol
-
 ?
1.2.98.1 formaldehyde + H2O + propionaldehyde cross-dismutation Pseudomonas putida formate + propanol
-
 ?
1.2.98.1 formaldehyde + H2O + propionaldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida formate + propanol
-
 ?
1.2.98.1 formaldehyde + H2O + propionaldehyde coupled oxidoreduction without addition of an electron acceptor Pseudomonas putida F61 formate + propanol
-
 ?
1.2.98.1 formaldehyde + H2O + propionaldehyde cross-dismutation Pseudomonas putida F61 formate + propanol
-
 ?
1.2.98.1 formaldehyde + H2O + propionaldehyde in general: RCHO + R'CHO = RCOOH + R'CH2OH Pseudomonas putida F61 formate + propanol
-
 ?
1.2.98.1 n-butanol + H2O + isobutyraldehyde in general: RCH2OH + R'CHO = RCHO + R'CH2OH, aliphatic alcohols with longer carbon chains than C-5, secondary alcohols, diols and aromatic alcohols and the corresponding aldehydes are no substrates Pseudomonas putida butyraldehyde + isobutanol
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.98.1 tetramer 4 * 55000, SDS-PAGE Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.98.1 0.0002
-
 Hg2+ noncompetitive inhibition Pseudomonas putida
1.2.98.1 0.52
-
 Semicarbazide noncompetitive inhibition Pseudomonas putida