Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kang, B.S.; Kim, Y.M.
    Cloning and molecular characterization of the genes for carbon monoxide dehydrogenase and localization of molybdopterin, flavin adenine dinucleotide, and iron-sulfur centers in the enzyme of Hydrogenophaga pseudoflava (1999), J. Bacteriol., 181, 5581-5590.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.5.3 genes cutM, cutS, and cutL, gene cluster cutMSL, DNA and amino acid sequence determination and anakysis, sequence comparisons, genetic organization Hydrogenophaga pseudoflava
1.2.7.4
-
 Hydrogenophaga pseudoflava

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.5.3 Fe2+
-
 Hydrogenophaga pseudoflava
1.2.5.3 iron-sulfur center two [2Fe-2S] clusters in the small subunit Hydrogenophaga pseudoflava
1.2.5.3 Molybdenum
-
 Hydrogenophaga pseudoflava
1.2.7.4 Iron 0.58, 0.67 and 1.96 atoms of iron per mol of large, medium- and small subunit, two iron-sulfur centers are associated with the small subunit Hydrogenophaga pseudoflava

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.5.3 17752
-
 x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation Hydrogenophaga pseudoflava
1.2.5.3 30694
-
 x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation Hydrogenophaga pseudoflava
1.2.5.3 87224
-
 x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation Hydrogenophaga pseudoflava
1.2.7.4 17752
-
 L2,M2,S2, 2 * 87224 + 2 * 30694 + 2 * 17752, deduced from gene sequence, SDS-PAGE Hydrogenophaga pseudoflava
1.2.7.4 30694
-
 L2,M2,S2, 2 * 87224 + 2 * 30694 + 2 * 17752, deduced from gene sequence, SDS-PAGE Hydrogenophaga pseudoflava
1.2.7.4 87224
-
 L2,M2,S2, 2 * 87224 + 2 * 30694 + 2 * 17752, deduced from gene sequence, SDS-PAGE Hydrogenophaga pseudoflava

Organism

EC Number Organism UniProt Comment Textmining
1.2.5.3 Hydrogenophaga pseudoflava P19913 and P19914 and P19915 large, medium, and small subunits; genes cutM, cutS, and cutL
-
1.2.5.3 Hydrogenophaga pseudoflava DSM 1084 P19913 and P19914 and P19915 large, medium, and small subunits; genes cutM, cutS, and cutL
-
1.2.7.4 Hydrogenophaga pseudoflava
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.3 native enzyme by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, followed by ultrafiltration, and gel filtration Hydrogenophaga pseudoflava
1.2.7.4
-
 Hydrogenophaga pseudoflava

Storage Stability

EC Number Storage Stability Organism
1.2.7.4 4°C, 1 month, 100% inactivation Hydrogenophaga pseudoflava

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.5.3 CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O
-
 Hydrogenophaga pseudoflava CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?
1.2.5.3 CO + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride + H2O
-
 Hydrogenophaga pseudoflava DSM 1084 CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.5.3 oligomer x * 87224, large subunit, + x * 30694, medium subunit, + x * 17752, small subunit, sequence calculation Hydrogenophaga pseudoflava
1.2.7.4 hexamer L2,M2,S2, 2 * 87224 + 2 * 30694 + 2 * 17752, deduced from gene sequence, SDS-PAGE Hydrogenophaga pseudoflava

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.5.3 30
-
 assay at Hydrogenophaga pseudoflava

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.5.3 7.2
-
 assay at Hydrogenophaga pseudoflava

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.5.3 FAD in the medium subunit Hydrogenophaga pseudoflava
1.2.5.3 molybdenum cofactor in the large subunit Hydrogenophaga pseudoflava
1.2.7.4 flavin adenine dinucleotide cofactor is released upon treatment with guanidine-HCl, SDS, heat or acid Hydrogenophaga pseudoflava
1.2.7.4 molybdopterin cytosine dinucleotide cofactor is released upon treatment with guanidine-HCl, SDS, heat or acid Hydrogenophaga pseudoflava

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.5.3 Hydrogenophaga pseudoflava large subunit, sequence calculation
-
 5.88
1.2.5.3 Hydrogenophaga pseudoflava small subunit, sequence calculation
-
 7.77
1.2.5.3 Hydrogenophaga pseudoflava medium subunit, sequence calculation
-
 8.43

General Information

EC Number General Information Comment Organism
1.2.5.3 evolution the sequences of CutMSL are highly conserved in CO-DHs and other molybdenum-containing hydroxylases Hydrogenophaga pseudoflava