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Literature summary extracted from

  • Grahame, D.A.
    Catalysis of acetyl-CoA cleavage and tetrahydrosarcinapterin methylation by a carbon monoxide dehydrogenase-corrinoid enzyme complex (1991), J. Biol. Chem., 266, 22227-22233.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.169 Fe corrinoid/iron-sulfur protein required Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.4 19700
-
 6 * 19700, alpha, + 6 * 84500, beta, + 6 * 63200, gamma, + 6 * 53000, delta, + 6 * 51400, epsilon, MW of the subunits of the carbon monoxide dehydrogenase-corrinoid enzyme complex, SDS-PAGE Methanosarcina barkeri
1.2.7.4 51400
-
 6 * 19700, alpha, + 6 * 84500, beta, + 6 * 63200, gamma, + 6 * 53000, delta, + 6 * 51400, epsilon, MW of the subunits of the carbon monoxide dehydrogenase-corrinoid enzyme complex, SDS-PAGE Methanosarcina barkeri
1.2.7.4 53000
-
 6 * 19700, alpha, + 6 * 84500, beta, + 6 * 63200, gamma, + 6 * 53000, delta, + 6 * 51400, epsilon, MW of the subunits of the carbon monoxide dehydrogenase-corrinoid enzyme complex, SDS-PAGE Methanosarcina barkeri
1.2.7.4 63200
-
 6 * 19700, alpha, + 6 * 84500, beta, + 6 * 63200, gamma, + 6 * 53000, delta, + 6 * 51400, epsilon, MW of the subunits of the carbon monoxide dehydrogenase-corrinoid enzyme complex, SDS-PAGE Methanosarcina barkeri
1.2.7.4 84500
-
 6 * 19700, alpha, + 6 * 84500, beta, + 6 * 63200, gamma, + 6 * 53000, delta, + 6 * 51400, epsilon, MW of the subunits of the carbon monoxide dehydrogenase-corrinoid enzyme complex, SDS-PAGE Methanosarcina barkeri
1.2.7.4 1600000
-
 carbon monoxide dehydrogenase-corrinoid enzyme complex, gel filtration Methanosarcina barkeri
2.3.1.169 19700
-
 alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE Methanosarcina barkeri
2.3.1.169 51400
-
 alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE Methanosarcina barkeri
2.3.1.169 53000
-
 alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE Methanosarcina barkeri
2.3.1.169 63200
-
 alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE Methanosarcina barkeri
2.3.1.169 84500
-
 alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE Methanosarcina barkeri
2.3.1.169 1600000
-
 gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.4 CO + H2O + electron acceptor Methanosarcina barkeri the carbon monoxide dehydrogenase-corrinoid enzyme complex catalyzes the methylation of tetrahydrosarcinapterin in a reaction involving net cleavage of acetyl-CoA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Methanosarcina barkeri
-
-
-
2.3.1.169 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.169
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + electron acceptor the carbon monoxide dehydrogenase-corrinoid enzyme complex catalyzes the methylation of tetrahydrosarcinapterin in a reaction involving net cleavage of acetyl-CoA Methanosarcina barkeri ?
-
 ?
1.2.7.4 tetrahydrosarcinapterin + acetyl-SCoA + H2O
-
 Methanosarcina barkeri methyltetrahydrosarcinapterin + CO2 + CoASH
-
 ?
2.3.1.169 CH3-CO-S-CoA + tetrahydrosarcinapterin + H2O the carbon monoxide dehydrogenase-corrinoid enzyme complex catalyzes the cleavage of acetyl-CoA, tetrahydrosarcinapterin functions as the methyl group acceptor, the major products of reaction are methyltetrahydrosarcinapterin and CO2, free CoA is identified as an additional product Methanosarcina barkeri CH3-tetrahydrosarcinapterin + CO2 + H+ + electron
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.4 oligomer 6 * 19700, alpha, + 6 * 84500, beta, + 6 * 63200, gamma, + 6 * 53000, delta, + 6 * 51400, epsilon, MW of the subunits of the carbon monoxide dehydrogenase-corrinoid enzyme complex, SDS-PAGE Methanosarcina barkeri
2.3.1.169 polymer alpha,beta,gamma,delta,epsilon, 6 * 19700 + 6 * 84500 + 6 * 63200 + 6 * 53000 + 6 * 51400, SDS-PAGE Methanosarcina barkeri