BRENDA - Enzyme Database show

Superoxide production by an unusual aldehyde oxidase in guinea pig granulocytes. Characterization and cytochemical localization

Badwey, J.A.; Robinson, J.M.; Karnovsky, M.J.; Karnovsky, M.L.; J. Biol. Chem. 256, 3479-3486 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.1
arsenite
-
Cavia porcellus
1.2.3.1
azide
slight inhibition
Cavia porcellus
1.2.3.1
ethanol
0.75 M, 40% inhibition
Cavia porcellus
1.2.3.1
KCN
-
Cavia porcellus
1.2.3.1
methanol
0.75 M, 62% inhibition
Cavia porcellus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.1
1.65
-
2-hydroxypyrimidine
-
Cavia porcellus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.3.1
Cavia porcellus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.3.1
partial purification
Cavia porcellus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.3.1
granulocyte
-
Cavia porcellus
-
Storage Stability
EC Number
Storage Stability
Organism
1.2.3.1
-20°C, 1 month, 100% activity retained
Cavia porcellus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
2-hydroxypyrimidine + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
uracil + H2O2
-
-
-
?
1.2.3.1
4-hydroxypyrimidine + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
?
-
-
-
?
1.2.3.1
acetaldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
acetic acid + H2O2
-
-
-
?
1.2.3.1
butanal + H2O + O2
ferricyanide as electron donor under aerobic conditions
390405
Cavia porcellus
butyric acid + H2O2
-
-
-
?
1.2.3.1
isobutyraldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
isobutyric acid + H2O2
-
-
-
?
1.2.3.1
isovaleraldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
isovaleric acid + H2O2
-
-
-
?
1.2.3.1
phenylacetaldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
phenylacetic acid + H2O2
-
-
-
?
1.2.3.1
quinoline + H2O + electron donor
ferricyanide as electron donor under aerobic conditions
390405
Cavia porcellus
?
-
-
-
?
1.2.3.1
salicylaldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
salicylic acid + H2O2
-
-
-
?
1.2.3.1
valeraldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
valeric acid + H2O2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.3.1
37
-
-
Cavia porcellus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.1
arsenite
-
Cavia porcellus
1.2.3.1
azide
slight inhibition
Cavia porcellus
1.2.3.1
ethanol
0.75 M, 40% inhibition
Cavia porcellus
1.2.3.1
KCN
-
Cavia porcellus
1.2.3.1
methanol
0.75 M, 62% inhibition
Cavia porcellus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.1
1.65
-
2-hydroxypyrimidine
-
Cavia porcellus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.3.1
partial purification
Cavia porcellus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.3.1
granulocyte
-
Cavia porcellus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.3.1
-20°C, 1 month, 100% activity retained
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
2-hydroxypyrimidine + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
uracil + H2O2
-
-
-
?
1.2.3.1
4-hydroxypyrimidine + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
?
-
-
-
?
1.2.3.1
acetaldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
acetic acid + H2O2
-
-
-
?
1.2.3.1
butanal + H2O + O2
ferricyanide as electron donor under aerobic conditions
390405
Cavia porcellus
butyric acid + H2O2
-
-
-
?
1.2.3.1
isobutyraldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
isobutyric acid + H2O2
-
-
-
?
1.2.3.1
isovaleraldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
isovaleric acid + H2O2
-
-
-
?
1.2.3.1
phenylacetaldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
phenylacetic acid + H2O2
-
-
-
?
1.2.3.1
quinoline + H2O + electron donor
ferricyanide as electron donor under aerobic conditions
390405
Cavia porcellus
?
-
-
-
?
1.2.3.1
salicylaldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
salicylic acid + H2O2
-
-
-
?
1.2.3.1
valeraldehyde + H2O + O2
ferricyanide as electron acceptor under aerobic conditions
390405
Cavia porcellus
valeric acid + H2O2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.3.1
37
-
-
Cavia porcellus