BRENDA - Enzyme Database show

Biochemical/spectroscopic characterization and preliminary x-ray analysis of a new aldehyde oxidoreductase isolated from Desulfovibrio desulfuricans ATCC 27774

Duarte, R.O.; Archer, M.; Dias, J.M.; Bursakov, S.; Huber, R.; Moura, I.; Romao, M.J.; Moura, J.J.G.; Biochem. Biophys. Res. Commun. 268, 745-749 (2000)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.2.3.1
2.8 A resolution, 98% complete
Desulfovibrio desulfuricans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.1
arsenite
65% inhibition at 20 mM
Desulfovibrio desulfuricans
1.2.3.1
Isopropanol
moderate inhibitor
Desulfovibrio desulfuricans
1.2.3.1
nitrate
moderate inhibitor
Desulfovibrio desulfuricans
1.2.3.1
phosphate
moderate inhibitor
Desulfovibrio desulfuricans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.1
0.012
-
propionaldehyde
-
Desulfovibrio desulfuricans
1.2.3.1
0.105
-
butanaldehyde
-
Desulfovibrio desulfuricans
1.2.3.1
0.151
-
benzaldehyde
-
Desulfovibrio desulfuricans
1.2.3.1
0.25
-
acetaldehyde
-
Desulfovibrio desulfuricans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.3.1
Fe2+
Fe/S center type I and Fe/S center type II
Desulfovibrio desulfuricans
1.2.3.1
Mo5+
-
Desulfovibrio desulfuricans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.3.1
100000
-
alpha2, 2 * 100000, SDS-PAGE
Desulfovibrio desulfuricans
1.2.3.1
200000
-
gel filtration
Desulfovibrio desulfuricans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.3.1
Desulfovibrio desulfuricans
-
ATCC 27774
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.3.1
-
Desulfovibrio desulfuricans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.3.1
0.8
-
2,6-dichlorophenol-indophenol under aerobic conditions as electron acceptor
Desulfovibrio desulfuricans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
acetaldehyde + H2O + O2
2,6-dichlorophenol-indophenol as electron acceptor
390375
Desulfovibrio desulfuricans
acetic acid + H2O2
-
-
-
?
1.2.3.1
benzaldehyde + H2O + O2
2,6-dichlorophenol-indophenol also as electron acceptor
390375
Desulfovibrio desulfuricans
benzoic acid + H2O2
-
-
-
?
1.2.3.1
butanal + H2O + O2
2,6-dichlorophenol-indophenol as electron acceptor
390375
Desulfovibrio desulfuricans
butyric acid + H2O2
-
-
-
?
1.2.3.1
propionaldehyde + H2O + O2
2,6-dichlorophenol-indophenol as electron acceptor
390375
Desulfovibrio desulfuricans
propionic acid + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.3.1
dimer
alpha2, 2 * 100000, SDS-PAGE
Desulfovibrio desulfuricans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.3.1
7.6
-
-
Desulfovibrio desulfuricans
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.2.3.1
4.9
9.5
-
Desulfovibrio desulfuricans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.3.1
molybdopterin cytosine dinucleotide
-
Desulfovibrio desulfuricans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.3.1
molybdopterin cytosine dinucleotide
-
Desulfovibrio desulfuricans
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.2.3.1
2.8 A resolution, 98% complete
Desulfovibrio desulfuricans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.1
arsenite
65% inhibition at 20 mM
Desulfovibrio desulfuricans
1.2.3.1
Isopropanol
moderate inhibitor
Desulfovibrio desulfuricans
1.2.3.1
nitrate
moderate inhibitor
Desulfovibrio desulfuricans
1.2.3.1
phosphate
moderate inhibitor
Desulfovibrio desulfuricans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.1
0.012
-
propionaldehyde
-
Desulfovibrio desulfuricans
1.2.3.1
0.105
-
butanaldehyde
-
Desulfovibrio desulfuricans
1.2.3.1
0.151
-
benzaldehyde
-
Desulfovibrio desulfuricans
1.2.3.1
0.25
-
acetaldehyde
-
Desulfovibrio desulfuricans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.3.1
Fe2+
Fe/S center type I and Fe/S center type II
Desulfovibrio desulfuricans
1.2.3.1
Mo5+
-
Desulfovibrio desulfuricans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.3.1
100000
-
alpha2, 2 * 100000, SDS-PAGE
Desulfovibrio desulfuricans
1.2.3.1
200000
-
gel filtration
Desulfovibrio desulfuricans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.3.1
-
Desulfovibrio desulfuricans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.3.1
0.8
-
2,6-dichlorophenol-indophenol under aerobic conditions as electron acceptor
Desulfovibrio desulfuricans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
acetaldehyde + H2O + O2
2,6-dichlorophenol-indophenol as electron acceptor
390375
Desulfovibrio desulfuricans
acetic acid + H2O2
-
-
-
?
1.2.3.1
benzaldehyde + H2O + O2
2,6-dichlorophenol-indophenol also as electron acceptor
390375
Desulfovibrio desulfuricans
benzoic acid + H2O2
-
-
-
?
1.2.3.1
butanal + H2O + O2
2,6-dichlorophenol-indophenol as electron acceptor
390375
Desulfovibrio desulfuricans
butyric acid + H2O2
-
-
-
?
1.2.3.1
propionaldehyde + H2O + O2
2,6-dichlorophenol-indophenol as electron acceptor
390375
Desulfovibrio desulfuricans
propionic acid + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.3.1
dimer
alpha2, 2 * 100000, SDS-PAGE
Desulfovibrio desulfuricans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.3.1
7.6
-
-
Desulfovibrio desulfuricans
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.2.3.1
4.9
9.5
-
Desulfovibrio desulfuricans