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Literature summary extracted from

  • Velasco-Garcia, R.; Mujica-Jimenez, C.; Mendoza-Hernandez, G.; Munoz-Clares, R.A.
    Rapid purification and properties of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa (1999), J. Bacteriol., 181, 1292-1300.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.2.1.8 at the optimum pH of 8.0 the enzyme is inactivated by dilution, it is stable at pH 6.5 even at very low concentrations Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 Betaine aldehyde
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 61000
-
 2 * 61000, SDS-PAGE Pseudomonas aeruginosa
1.2.1.8 139000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa enzyme catalyzes the last, irreversible step in the synthesis of the osmoprotectant glycine betaine from choline, also obligatory step in the assimilation of carbon and nitrogen when bacteria are growing in choline or choline precursors betaine + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 rapid purification Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.8 146.3
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 4-aminobutyraldehyde + NAD+ + H2O no activity Pseudomonas aeruginosa 4-aminobutyrate + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O preferentially uses NADP+ over NAD+ Pseudomonas aeruginosa betaine + NADH
-
 ir
1.2.1.8 betaine aldehyde + NAD+ + H2O enzyme catalyzes the last, irreversible step in the synthesis of the osmoprotectant glycine betaine from choline, also obligatory step in the assimilation of carbon and nitrogen when bacteria are growing in choline or choline precursors Pseudomonas aeruginosa betaine + NADH
-
 ir
1.2.1.8 betaine aldehyde + NADP+ + H2O preferentially uses NADP+ over NAD+ Pseudomonas aeruginosa betaine + NADPH
-
 ?
1.2.1.8 additional information no activity with 3-dimethylsulfoniopropionaldehyde Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.8 dimer 2 * 61000, SDS-PAGE Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.8 40
-
 loss of activity, activity is regained, when the heated enzyme is cooled to 30°C or lower Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 8
-
 Tris-HCl buffer Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.8 7 9.5 pH 7.0: about 50% of maximal activity, pH 9.5: about 75% of maximal activity Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Pseudomonas aeruginosa
1.2.1.8 NADP+ preferentially uses NADP+ over NAD+ Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.8 4.6
-
 Betaine aldehyde
-
 Pseudomonas aeruginosa