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Literature summary extracted from
- Human liver fatty aldehyde dehydrogenase: microsomal localization, purification, and biochemical characterization (1997), Biochim. Biophys. Acta, 1335, 99-110.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.48 | alpha,p-dibromoacetophenone | 0.03 mM causes 100% inhibition | Homo sapiens |  |
| 1.2.1.48 | Chloral hydrate | 25 mM causes 29% inhibition | Homo sapiens | |
| 1.2.1.48 | Disulfiram | 0.065 mM causes 100% inhibition | Homo sapiens | |
| 1.2.1.48 | iodoacetamide | 10 mM causes 96% inhibition | Homo sapiens | |
| 1.2.1.48 | iodoacetate | 10 mM causes 92% inhibition | Homo sapiens | |
| 1.2.1.48 | N-ethylmaleimide | 2 mM causes 43% inhibition | Homo sapiens | |
| 1.2.1.48 | p-chloromercuribenzoate | 0.01 mM causes complete inhibition | Homo sapiens | |
| 1.2.1.48 | p-hydroxyacetophenone | 0.25 mM causes 46% inhibition | Homo sapiens | |
| 1.2.1.48 | potassium cyanide | 1 mM causes 33% inhibition | Homo sapiens | |
| 1.2.1.48 | Zn2+ | 1 mM causes 47% inhibition | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.48 | 0.006 | - |
cis,cis-9,12-octadecadienal | - |
Homo sapiens | |
| 1.2.1.48 | 0.006 | - |
dihydrophytal | - |
Homo sapiens | |
| 1.2.1.48 | 0.011 | - |
cis-9-octadecenal | - |
Homo sapiens | |
| 1.2.1.48 | 0.012 | - |
cis-9-hexadecenal | - |
Homo sapiens | |
| 1.2.1.48 | 0.018 | - |
tetraeicosanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.019 | - |
dodecanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.021 | - |
octadecanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.023 | - |
decanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.023 | - |
Tetradecanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.028 | - |
Pentadecanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.032 | - |
octanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.032 | - |
hexadecanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.036 | - |
behenic aldehyde | - |
Homo sapiens | |
| 1.2.1.48 | 0.038 | - |
arachidic aldehyde | - |
Homo sapiens | |
| 1.2.1.48 | 0.05 | - |
hexanal | - |
Homo sapiens | |
| 1.2.1.48 | 0.28 | - |
NAD+ | reference substrate octadecanal | Homo sapiens | |
| 1.2.1.48 | 0.8 | - |
crotonaldehyde | - |
Homo sapiens | |
| 1.2.1.48 | 1.3 | - |
propionaldehyde | - |
Homo sapiens | |
| 1.2.1.48 | 1.44 | - |
benzaldehyde | - |
Homo sapiens | |
| 1.2.1.48 | 1.7 | - |
Glutaraldehyde | - |
Homo sapiens | |
| 1.2.1.48 | 2.5 | - |
acetaldehyde | - |
Homo sapiens | |
| 1.2.1.48 | 8.7 | - |
NADP+ | reference substrate octadecanal | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.2.1.48 | microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.48 | 54000 | - |
x * 54000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.48 | long-chain aldehyde + NAD+ | Homo sapiens | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.48 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.48 | using chromatography on columns consisting of omega-aminohexyl-agarose and 5'-AMP-Sepharose 4B | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.2.1.48 | liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.48 | 33.3 | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.2.1.48 | -20°C, 25 mM potassium phosphate, pH 7.5, 5% glycerol, 0.2 mM NAD+, 3 months, retains more than 80% of its original activity | Homo sapiens |
| 1.2.1.48 | 4°C, 25 mM potassium phosphate, pH 7.5, 5% glycerol, 0.2 mM NAD+, 1 month, retains 67% of its original activity | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.48 | acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? | |
| 1.2.1.48 | arachidic aldehyde + NAD+ + H2O | - |
Homo sapiens | arachidic acid + NADH | - |
? | |
| 1.2.1.48 | behenic aldehyde + NAD+ + H2O | - |
Homo sapiens | behenic acid + NADH | - |
? | |
| 1.2.1.48 | benzaldehyde + NAD+ + H2O | - |
Homo sapiens | benzoate + NADH + H+ | - |
? | |
| 1.2.1.48 | cis,cis-9,12-octadecadienal + NAD+ + H2O | - |
Homo sapiens | cis,cis-9,12-octadecadienoic acid + NADH | - |
? | |
| 1.2.1.48 | cis-9-hexadecenal + NAD+ + H2O | - |
Homo sapiens | cis-9-hexadecenoic acid + NADH | - |
? | |
| 1.2.1.48 | cis-9-octadecenal + NAD+ + H2O | - |
Homo sapiens | cis-9-octadecenoic acid + NADH | - |
? | |
| 1.2.1.48 | crotonaldehyde + NAD+ + H2O | - |
Homo sapiens | crotonic acid + NADH | - |
? | |
| 1.2.1.48 | decanal + NAD+ + H2O | - |
Homo sapiens | decanoic acid + NADH | - |
? | |
| 1.2.1.48 | dihydrophytal + NAD+ + H2O | - |
Homo sapiens | (3R,S,7R,11R)-3,7,11,15-tetramethylhexadecanoic acid + NADH | - |
? | |
| 1.2.1.48 | dodecanal + NAD+ + H2O | - |
Homo sapiens | dodecanoic acid + NADH | - |
? | |
| 1.2.1.48 | glutaraldehyde + NAD+ | - |
Homo sapiens | glutaric acid + NADH | - |
? | |
| 1.2.1.48 | hexadecanal + NAD+ + H2O | - |
Homo sapiens | hexadecanoic acid + NADH | - |
? | |
| 1.2.1.48 | hexanal + NAD+ + H2O | - |
Homo sapiens | hexanoate + NADH + H+ | - |
? | |
| 1.2.1.48 | long-chain aldehyde + NAD+ | - |
Homo sapiens | ? | - |
? | |
| 1.2.1.48 | long-chain aldehyde + NAD+ + H2O | - |
Homo sapiens | long-chain acid anion + NADH | - |
? | |
| 1.2.1.48 | additional information | poor substrates: acetaldehyde, propionaldehyde, crotonaldehyde, glutaraldehyde, benzaldehyde, retinaldehyde | Homo sapiens | ? | - |
? | |
| 1.2.1.48 | octadecanal + NAD+ + H2O | - |
Homo sapiens | octadecanoic acid + NADH | - |
? | |
| 1.2.1.48 | octadecanal + NADP+ | - |
Homo sapiens | octadecanoic acid + NADPH + H+ | - |
r | |
| 1.2.1.48 | octanal + NAD+ + H2O | - |
Homo sapiens | octanoic acid + NADH + H+ | - |
? | |
| 1.2.1.48 | pentadecanal + NAD+ + H2O | - |
Homo sapiens | pentadecanoic acid + NADH | - |
? | |
| 1.2.1.48 | propionaldehyde + NAD+ + H2O | - |
Homo sapiens | propionic acid + NADH | - |
? | |
| 1.2.1.48 | tetradecanal + NAD+ + H2O | - |
Homo sapiens | tetradecanoic acid + NADH | - |
? | |
| 1.2.1.48 | tetraeicosanal + NAD+ + H2O | - |
Homo sapiens | tetraeicosanoic acid + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.1.48 | ? | x * 54000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.48 | 47 | - |
5 min, the purified enzyme retains only 13% of its original activity | Homo sapiens |
| 1.2.1.48 | 52 | - |
5 min, 100% loss of activity | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.48 | 9.8 | - |
in glycine buffer | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.48 | NAD+ | the enzyme has a much greater specificity for NAD+ as cofactor than NADP+ | Homo sapiens | |
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