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Literature summary extracted from

  • MacKintosh, R.W.; Fewson, C.A.
    Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II from Acinetobacter calcoaceticus (1987), Biochem. Soc. Trans., 15, 1048-1049.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.2.1.28 dithiothreitol stabilizes Acinetobacter calcoaceticus
1.2.1.28 MgCl2 stabilizes Acinetobacter calcoaceticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.28 4-chloromercuribenzoate
-
 Acinetobacter calcoaceticus
1.2.1.28 benzaldehyde inhibition at concentrations above 0.01 mM, benzaldehyde dehydrogenase II Acinetobacter calcoaceticus
1.2.1.28 iodoacetamide
-
 Acinetobacter calcoaceticus
1.2.1.28 iodoacetate
-
 Acinetobacter calcoaceticus
1.2.1.28 N-ethylmaleimide
-
 Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.28 0.00068
-
 benzaldehyde
-
 Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.28 55000
-
 4 * 55000, dehydrogenase II, SDS-PAGE Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.28 benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.28 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.28 dehydrogenase II Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase II exhibits esterase activity with 4-nitrophenyl acetate Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ?
1.2.1.28 hexan-1-al + NAD+ + H2O
-
 Acinetobacter calcoaceticus hexanoate + NADH
-
 ?
1.2.1.28 octan-1-al + NAD+ + H2O
-
 Acinetobacter calcoaceticus octanoate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.28 tetramer 4 * 55000, dehydrogenase II, SDS-PAGE Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.28 NAD+
-
 Acinetobacter calcoaceticus