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Literature summary extracted from

  • Bannerjee, D.; Sanders, L.E.; Sokatch, J.R.
    Properties of purified methylmalonate semialdehyde dehydrogenase of Pseudomonas aeruginosa (1970), J. Biol. Chem., 245, 1828-1835.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.27 0.019
-
 2-Methyl-3-oxopropanoate
-
 Pseudomonas aeruginosa
1.2.1.27 0.022
-
 CoA
-
 Pseudomonas aeruginosa
1.2.1.27 0.082
-
 NAD+
-
 Pseudomonas aeruginosa
1.2.1.27 0.54
-
 2-mercaptoethanol
-
 Pseudomonas aeruginosa
1.2.1.27 6.1
-
 propanal
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.27 58000
-
 2 * 58000, SDS-PAGE Pseudomonas aeruginosa
1.2.1.27 69000
-
 2 * 69000, gel filtration in presence of SDS Pseudomonas aeruginosa
1.2.1.27 132000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+ Pseudomonas aeruginosa
-
 propanoyl-CoA + CO2 + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.27 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.27
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.27 5
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+
-
 Pseudomonas aeruginosa propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+ other acyl acceptor: 2-mercaptoethanol Pseudomonas aeruginosa propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 propanal + 2-mercaptoethanol + NAD+
-
 Pseudomonas aeruginosa ? + NADH
-
 ir
1.2.1.27 propanal + CoA + NAD+
-
 Pseudomonas aeruginosa propanoyl-CoA + NADH
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.1.27 dimer 2 * 58000, SDS-PAGE Pseudomonas aeruginosa
1.2.1.27 dimer 2 * 69000, gel filtration in presence of SDS Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.27 CoA
-
 Pseudomonas aeruginosa
1.2.1.27 NAD+
-
 Pseudomonas aeruginosa