BRENDA - Enzyme Database

Separation and characterization of glycolaldehyde dehydrogenase isozymes in Escherichia coli B

Tani, Y.; Morita, H.; Nishise, H.; Ogata, K.; Agric. Biol. Chem. 42, 63-68 (1978)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.21
Al3+
1 mM, isozyme A, 26% inhibition
Escherichia coli
1.2.1.21
As3+
1 mM, isozyme A, 42% inhibition, isozyme B, 11% inhibition, isozyme C, 55% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Ca2+
1 mM, isozyme A, 42% inhibition, isozyme B, 32% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Co2+
1 mM, isozyme A, 71% inhibition, isozyme B, 51% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Fe2+
; 1 mM, isozyme A, 18% inhibition
Escherichia coli
1.2.1.21
HgCl2
1 mM, complete inhibition of isozymes A, B, and C
Escherichia coli
1.2.1.21
KCN
isozymes A and C
Escherichia coli
1.2.1.21
Mg2+
1 mM, isozyme A, 33% inhibition, isozyme B, 54% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Mn2+
1 mM, isozyme A, 20% inhibition, isozyme C, 44% inhibition
Escherichia coli
1.2.1.21
monoiodoacetate
1 mM, isozymes A, 83% inhibition, isozyme C, 100% inhibition
Escherichia coli
1.2.1.21
MoO22-
1 mM, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Zn2+
1 mM, isozyme A, 30% inhibition
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.21
68000
-
isozyme B, gel filtration
Escherichia coli
1.2.1.21
225000
-
isozyme A, gel filtration
Escherichia coli
1.2.1.21
345000
-
isozyme C, gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.21
Escherichia coli
-
-
-
1.2.1.21
Escherichia coli B WG1
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.21
partial: isozymes A, B and C
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.21
2.7
-
isozyme B
Escherichia coli
1.2.1.21
2.75
-
isozyme C
Escherichia coli
1.2.1.21
3.66
-
isozyme A
Escherichia coli
1.2.1.21
6.38
-
isozyme A from pyridoxine auxotroph strain WG3
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.21
-20░C
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.21
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli
acetate + NADH + H+
-
390215
Escherichia coli
r
1.2.1.21
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli B WG1
acetate + NADH + H+
-
390215
Escherichia coli B WG1
r
1.2.1.21
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli
benzoate + NADH + H+
-
390215
Escherichia coli
-
1.2.1.21
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli B WG1
benzoate + NADH + H+
-
390215
Escherichia coli B WG1
-
1.2.1.21
betaine + NADH
isozyme A 139% of activity with glycolate, isozyme B, 76%
390215
Escherichia coli
betaine aldehyde + NAD+ + H2O
glucono-delta-lactone is not used as substrate
390215
Escherichia coli
?
1.2.1.21
formate + NADH
isozyme A, 163% of activity with glycolate
390215
Escherichia coli
methanal + NAD+
-
390215
Escherichia coli
?
1.2.1.21
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli
glycolate + NADH
-
390215
Escherichia coli
r
1.2.1.21
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli B WG1
glycolate + NADH
-
390215
Escherichia coli B WG1
r
1.2.1.21
glyoxylate + NADH
isozyme A, 417% of activity with glycolate, isozyme B 714%, isozyme C 150%
390215
Escherichia coli
glyoxal + NAD+
-
390215
Escherichia coli
?
1.2.1.21
malonate + NADH
isozyme A, 83% of activity with glycolate
390215
Escherichia coli
malonic semialdehyde + NAD+ + H2O
-
-
-
?
1.2.1.21
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli
glyoxylate + NAD+
-
390215
Escherichia coli
?
1.2.1.21
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli B WG1
glyoxylate + NAD+
-
390215
Escherichia coli B WG1
?
1.2.1.21
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli
succinic semialdehyde + NAD+ + H2O
-
-
-
?
1.2.1.21
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli B WG1
succinic semialdehyde + NAD+ + H2O
-
-
-
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.2.1.21
20
-
isozyme A
Escherichia coli
1.2.1.21
30
-
isozyme A from strain WG3
Escherichia coli
1.2.1.21
45
-
isozymes B and C
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.21
5
-
glycolate reduction, isozyme A, B
Escherichia coli
1.2.1.21
5.4
-
isozyme A, glycolaldehyde oxidation
Escherichia coli
1.2.1.21
6
-
glycolate reduction, isozyme C
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.21
NADP+
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.21
NADP+
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.21
Al3+
1 mM, isozyme A, 26% inhibition
Escherichia coli
1.2.1.21
As3+
1 mM, isozyme A, 42% inhibition, isozyme B, 11% inhibition, isozyme C, 55% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Ca2+
1 mM, isozyme A, 42% inhibition, isozyme B, 32% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Co2+
1 mM, isozyme A, 71% inhibition, isozyme B, 51% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Fe2+
; 1 mM, isozyme A, 18% inhibition
Escherichia coli
1.2.1.21
HgCl2
1 mM, complete inhibition of isozymes A, B, and C
Escherichia coli
1.2.1.21
KCN
isozymes A and C
Escherichia coli
1.2.1.21
Mg2+
1 mM, isozyme A, 33% inhibition, isozyme B, 54% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Mn2+
1 mM, isozyme A, 20% inhibition, isozyme C, 44% inhibition
Escherichia coli
1.2.1.21
monoiodoacetate
1 mM, isozymes A, 83% inhibition, isozyme C, 100% inhibition
Escherichia coli
1.2.1.21
MoO22-
1 mM, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
1.2.1.21
Zn2+
1 mM, isozyme A, 30% inhibition
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.21
68000
-
isozyme B, gel filtration
Escherichia coli
1.2.1.21
225000
-
isozyme A, gel filtration
Escherichia coli
1.2.1.21
345000
-
isozyme C, gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.21
partial: isozymes A, B and C
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.21
2.7
-
isozyme B
Escherichia coli
1.2.1.21
2.75
-
isozyme C
Escherichia coli
1.2.1.21
3.66
-
isozyme A
Escherichia coli
1.2.1.21
6.38
-
isozyme A from pyridoxine auxotroph strain WG3
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.21
-20░C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.21
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli
acetate + NADH + H+
-
390215
Escherichia coli
r
1.2.1.21
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli B WG1
acetate + NADH + H+
-
390215
Escherichia coli B WG1
r
1.2.1.21
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli
benzoate + NADH + H+
-
390215
Escherichia coli
-
1.2.1.21
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli B WG1
benzoate + NADH + H+
-
390215
Escherichia coli B WG1
-
1.2.1.21
betaine + NADH
isozyme A 139% of activity with glycolate, isozyme B, 76%
390215
Escherichia coli
betaine aldehyde + NAD+ + H2O
glucono-delta-lactone is not used as substrate
390215
Escherichia coli
?
1.2.1.21
formate + NADH
isozyme A, 163% of activity with glycolate
390215
Escherichia coli
methanal + NAD+
-
390215
Escherichia coli
?
1.2.1.21
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli
glycolate + NADH
-
390215
Escherichia coli
r
1.2.1.21
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli B WG1
glycolate + NADH
-
390215
Escherichia coli B WG1
r
1.2.1.21
glyoxylate + NADH
isozyme A, 417% of activity with glycolate, isozyme B 714%, isozyme C 150%
390215
Escherichia coli
glyoxal + NAD+
-
390215
Escherichia coli
?
1.2.1.21
malonate + NADH
isozyme A, 83% of activity with glycolate
390215
Escherichia coli
malonic semialdehyde + NAD+ + H2O
-
-
-
?
1.2.1.21
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli
glyoxylate + NAD+
-
390215
Escherichia coli
?
1.2.1.21
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli B WG1
glyoxylate + NAD+
-
390215
Escherichia coli B WG1
?
1.2.1.21
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli
succinic semialdehyde + NAD+ + H2O
-
-
-
?
1.2.1.21
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli B WG1
succinic semialdehyde + NAD+ + H2O
-
-
-
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.2.1.21
20
-
isozyme A
Escherichia coli
1.2.1.21
30
-
isozyme A from strain WG3
Escherichia coli
1.2.1.21
45
-
isozymes B and C
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.21
5
-
glycolate reduction, isozyme A, B
Escherichia coli
1.2.1.21
5.4
-
isozyme A, glycolaldehyde oxidation
Escherichia coli
1.2.1.21
6
-
glycolate reduction, isozyme C
Escherichia coli