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Literature summary extracted from

  • Paris, S.; Wessel, P.M.; Dumas, R.
    Overproduction, purification, and characterization of recombinant aspartate semialdehyde dehydrogenase from Arabidopsis thaliana (2002), Protein Expr. Purif., 24, 99-104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 cloning and recombinant protein overproduction with Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.11 0.0929
-
 NADPH at 0.15 mM aspartyl phosphate Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.11 36000
-
 2 * 36000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH Arabidopsis thaliana
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Arabidopsis thaliana Q9FVC4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11 using anionic exchange chromatography on DEAE EMD 650 column and Q-Sepharose column Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.11 26
-
-
 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
1.2.1.11 -80°C, no noticeable loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH
-
 Arabidopsis thaliana L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH + H+
-
 Arabidopsis thaliana L-aspartate 4-semialdehyde + phosphate + NADP+
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Arabidopsis thaliana L-4-aspartyl phosphate + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.11 dimer 2 * 36000, SDS-PAGE Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.11 15.6
-
 L-4-aspartyl phosphate
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADPH
-
 Arabidopsis thaliana