BRENDA - Enzyme Database show

Overproduction, purification, and characterization of recombinant aspartate semialdehyde dehydrogenase from Arabidopsis thaliana

Paris, S.; Wessel, P.M.; Dumas, R.; Protein Expr. Purif. 24, 99-104 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
cloning and recombinant protein overproduction with Escherichia coli
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.0929
-
NADPH
at 0.15 mM aspartyl phosphate
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
36000
-
2 * 36000, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Arabidopsis thaliana
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Arabidopsis thaliana
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Arabidopsis thaliana
Q9FVC4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
using anionic exchange chromatography on DEAE EMD 650 column and Q-Sepharose column
Arabidopsis thaliana
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
26
-
-
Arabidopsis thaliana
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.11
-80░C, no noticeable loss of activity
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390203
Arabidopsis thaliana
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390203
Arabidopsis thaliana
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390203
Arabidopsis thaliana
L-4-aspartyl phosphate + NADPH
-
390203
Arabidopsis thaliana
r
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
dimer
2 * 36000, SDS-PAGE
Arabidopsis thaliana
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
15.6
-
aspartyl phosphate
-
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
cloning and recombinant protein overproduction with Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.0929
-
NADPH
at 0.15 mM aspartyl phosphate
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
36000
-
2 * 36000, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Arabidopsis thaliana
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Arabidopsis thaliana
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
using anionic exchange chromatography on DEAE EMD 650 column and Q-Sepharose column
Arabidopsis thaliana
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
26
-
-
Arabidopsis thaliana
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.11
-80░C, no noticeable loss of activity
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390203
Arabidopsis thaliana
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390203
Arabidopsis thaliana
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390203
Arabidopsis thaliana
L-4-aspartyl phosphate + NADPH
-
390203
Arabidopsis thaliana
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
dimer
2 * 36000, SDS-PAGE
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
15.6
-
aspartyl phosphate
-
Arabidopsis thaliana