BRENDA - Enzyme Database show

Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganisms

Moore, R.A.; Bocik, W.E.; Viola, R.E.; Protein Expr. Purif. 25, 189-194 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
expressed as inclusion bodies with an N-terminal hexahistidine tag in pET28b vector or subcloned into pET41 vector with NdeI/SalI
Helicobacter pylori
1.2.1.11
expressed in Escherichia coli
Haemophilus influenzae
1.2.1.11
expressed in Escherichia coli
Pseudomonas aeruginosa
1.2.1.11
expressed in Escherichia coli, the genome contains two genes for the enzyme
Vibrio cholerae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.12
-
L-aspartate 4-semialdehyde
-
Pseudomonas aeruginosa
1.2.1.11
0.13
-
NADP+
-
Pseudomonas aeruginosa
1.2.1.11
0.15
-
NADP+
-
Haemophilus influenzae
1.2.1.11
0.16
-
L-aspartate 4-semialdehyde
gene II
Vibrio cholerae
1.2.1.11
0.19
-
L-aspartate 4-semialdehyde
gene I
Vibrio cholerae
1.2.1.11
0.24
-
L-aspartate 4-semialdehyde
-
Haemophilus influenzae
1.2.1.11
0.32
-
NADP+
gene I
Vibrio cholerae
1.2.1.11
0.36
-
NADP+
gene II
Vibrio cholerae
1.2.1.11
1.1
-
phosphate
gene I
Vibrio cholerae
1.2.1.11
1.5
-
phosphate
-
Pseudomonas aeruginosa
1.2.1.11
1.6
-
phosphate
-
Haemophilus influenzae
1.2.1.11
22
-
phosphate
gene II
Vibrio cholerae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Haemophilus influenzae
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Haemophilus influenzae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Pseudomonas aeruginosa
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Pseudomonas aeruginosa
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Helicobacter pylori
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Helicobacter pylori
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Vibrio cholerae
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Vibrio cholerae
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Haemophilus influenzae
-
-
-
1.2.1.11
Helicobacter pylori
O25801
-
-
1.2.1.11
Pseudomonas aeruginosa
Q51344
-
-
1.2.1.11
Vibrio cholerae
P23247
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
-
Helicobacter pylori
1.2.1.11
using ammonium sulfate precipitation and column chromatography on Q Sepharose XL and Procion Red-A
Haemophilus influenzae
1.2.1.11
using column chromatography on Q Sepharose XL and omega-aminohexyl-agarose for the purification of V. cholerae I and column chromatography on Q Sepharose XL for the purification of V. cholerae II
Vibrio cholerae
1.2.1.11
using column chromatography on Q Sepharose XL and phenyl-Sepharose
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
-
Haemophilus influenzae
1.2.1.11
additional information
-
-
Pseudomonas aeruginosa
1.2.1.11
additional information
-
-
Vibrio cholerae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Haemophilus influenzae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Haemophilus influenzae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Pseudomonas aeruginosa
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Pseudomonas aeruginosa
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Helicobacter pylori
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Helicobacter pylori
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Vibrio cholerae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Vibrio cholerae
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Haemophilus influenzae
L-4-aspartyl phosphate + NADPH
-
390202
Haemophilus influenzae
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Pseudomonas aeruginosa
L-4-aspartyl phosphate + NADPH
-
390202
Pseudomonas aeruginosa
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Helicobacter pylori
L-4-aspartyl phosphate + NADPH
-
390202
Helicobacter pylori
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Vibrio cholerae
L-4-aspartyl phosphate + NADPH
-
390202
Vibrio cholerae
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
58
-
L-aspartate 4-semialdehyde
gene II
Vibrio cholerae
1.2.1.11
120
-
L-aspartate 4-semialdehyde
gene I
Vibrio cholerae
1.2.1.11
160
-
L-aspartate 4-semialdehyde
-
Pseudomonas aeruginosa
1.2.1.11
330
-
L-aspartate 4-semialdehyde
-
Haemophilus influenzae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Pseudomonas aeruginosa
1.2.1.11
NADP+
-
Helicobacter pylori
1.2.1.11
NADP+
-
Haemophilus influenzae
1.2.1.11
NADP+
-
Vibrio cholerae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
expressed as inclusion bodies with an N-terminal hexahistidine tag in pET28b vector or subcloned into pET41 vector with NdeI/SalI
Helicobacter pylori
1.2.1.11
expressed in Escherichia coli
Haemophilus influenzae
1.2.1.11
expressed in Escherichia coli
Pseudomonas aeruginosa
1.2.1.11
expressed in Escherichia coli, the genome contains two genes for the enzyme
Vibrio cholerae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Pseudomonas aeruginosa
1.2.1.11
NADP+
-
Haemophilus influenzae
1.2.1.11
NADP+
-
Helicobacter pylori
1.2.1.11
NADP+
-
Vibrio cholerae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.12
-
L-aspartate 4-semialdehyde
-
Pseudomonas aeruginosa
1.2.1.11
0.13
-
NADP+
-
Pseudomonas aeruginosa
1.2.1.11
0.15
-
NADP+
-
Haemophilus influenzae
1.2.1.11
0.16
-
L-aspartate 4-semialdehyde
gene II
Vibrio cholerae
1.2.1.11
0.19
-
L-aspartate 4-semialdehyde
gene I
Vibrio cholerae
1.2.1.11
0.24
-
L-aspartate 4-semialdehyde
-
Haemophilus influenzae
1.2.1.11
0.32
-
NADP+
gene I
Vibrio cholerae
1.2.1.11
0.36
-
NADP+
gene II
Vibrio cholerae
1.2.1.11
1.1
-
phosphate
gene I
Vibrio cholerae
1.2.1.11
1.5
-
phosphate
-
Pseudomonas aeruginosa
1.2.1.11
1.6
-
phosphate
-
Haemophilus influenzae
1.2.1.11
22
-
phosphate
gene II
Vibrio cholerae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Haemophilus influenzae
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Haemophilus influenzae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Pseudomonas aeruginosa
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Pseudomonas aeruginosa
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Helicobacter pylori
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Helicobacter pylori
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Vibrio cholerae
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Vibrio cholerae
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
-
Helicobacter pylori
1.2.1.11
using ammonium sulfate precipitation and column chromatography on Q Sepharose XL and Procion Red-A
Haemophilus influenzae
1.2.1.11
using column chromatography on Q Sepharose XL and omega-aminohexyl-agarose for the purification of V. cholerae I and column chromatography on Q Sepharose XL for the purification of V. cholerae II
Vibrio cholerae
1.2.1.11
using column chromatography on Q Sepharose XL and phenyl-Sepharose
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
-
Haemophilus influenzae
1.2.1.11
additional information
-
-
Pseudomonas aeruginosa
1.2.1.11
additional information
-
-
Vibrio cholerae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Haemophilus influenzae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Haemophilus influenzae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Pseudomonas aeruginosa
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Pseudomonas aeruginosa
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Helicobacter pylori
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Helicobacter pylori
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
-
390202
Vibrio cholerae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Vibrio cholerae
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Haemophilus influenzae
L-4-aspartyl phosphate + NADPH
-
390202
Haemophilus influenzae
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Pseudomonas aeruginosa
L-4-aspartyl phosphate + NADPH
-
390202
Pseudomonas aeruginosa
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Helicobacter pylori
L-4-aspartyl phosphate + NADPH
-
390202
Helicobacter pylori
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390202
Vibrio cholerae
L-4-aspartyl phosphate + NADPH
-
390202
Vibrio cholerae
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
58
-
L-aspartate 4-semialdehyde
gene II
Vibrio cholerae
1.2.1.11
120
-
L-aspartate 4-semialdehyde
gene I
Vibrio cholerae
1.2.1.11
160
-
L-aspartate 4-semialdehyde
-
Pseudomonas aeruginosa
1.2.1.11
330
-
L-aspartate 4-semialdehyde
-
Haemophilus influenzae