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Literature summary extracted from

  • Zhang, W.W.; Jiang, W.H.; Zhao, G.P.; Yang, Y.L.; Chiao, J.S.
    Expression in Escherichia coli, purification and kinetic analysis of the aspartokinase and aspartate semialdehyde dehydrogenase from the rifamycin SV-producing Amycolatopsis mediterranei U32 (2000), Appl. Microbiol. Biotechnol., 54, 52-58.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expressed in Escherichia coli Amycolatopsis mediterranei U32
2.7.2.4 operon encoding aspartokinase cloned and sequenced, introduced into auxotrophic Escherichia coli CGSC5074 and Escherichia coli X6118 and functionally expressed Amycolatopsis mediterranei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.11 L-isoleucine at 1 mM: 18% repression of enzyme synthesis, at 5 mM: 62% repression of enzyme synthesis Amycolatopsis mediterranei U32
1.2.1.11 L-lysine at 1 mM: 22% repression of the enzyme synthesis, at 5 mM: 44% repression of the enzyme synthesis, at 10 mM: 28% inhibition of the enzyme activity assayed in the reverse reaction Amycolatopsis mediterranei U32
1.2.1.11 L-methionine at 1 mM: 40% repression of the enzyme synthesis, at 5 mM: 62% repression of the enzyme synthesis Amycolatopsis mediterranei U32
1.2.1.11 L-threonine at 1 mM: 25% repression of the enzyme synthesis, at 5 mM: 43% repression of the enzyme synthesis, at 10 mM: 37% inhibition of the enzyme activity assayed in the reverse reaction Amycolatopsis mediterranei U32
2.7.2.4 L-isoleucine repression of aspartokinase gene transcription Amycolatopsis mediterranei
2.7.2.4 L-lysine repression of aspartokinase gene transcription Amycolatopsis mediterranei
2.7.2.4 L-threonine repression of aspartokinase gene transcription Amycolatopsis mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.11 0.73
-
 NADP+
-
 Amycolatopsis mediterranei U32
1.2.1.11 1.25
-
 L-aspartate 4-semialdehyde
-
 Amycolatopsis mediterranei U32
2.7.2.4 2.3
-
 ATP
-
 Amycolatopsis mediterranei
2.7.2.4 3.4
-
 L-aspartate
-
 Amycolatopsis mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.11 37000
-
 x * 37000, SDS-PAGE Amycolatopsis mediterranei U32

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Amycolatopsis mediterranei U32
-
-
-
2.7.2.4 Amycolatopsis mediterranei
-
 U32
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11
-
 Amycolatopsis mediterranei U32
2.7.2.4 recombinant enzyme, expressed in E. coli Amycolatopsis mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.11 0.0029
-
 of the recombinant in the crude extracts of Escherichia coli Amycolatopsis mediterranei U32

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Amycolatopsis mediterranei U32 L-4-aspartyl phosphate + NADPH
-
 ?
2.7.2.4 ATP + L-aspartate
-
 Amycolatopsis mediterranei ADP + 4-phospho-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.11 ? x * 37000, SDS-PAGE Amycolatopsis mediterranei U32

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADP+
-
 Amycolatopsis mediterranei U32