BRENDA - Enzyme Database show

Adenosine 5-triphosphate induced cold inactivation of yeast aspartic -semialdehyde dehydrogenase

Holland, M.J.; Westhead, E.W.; Biochemistry 12, 2270-2275 (1973)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.2.1.11
adenosine 5'-triphosphate, time dependent inactivation at 0°C
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
Adenosine 5'-triphosphate
causes a time-dependent inactivation at a concentration of 3.5 mM, 0°C, pH 6.5 and 2 mM dithiothreitol, inactivation can be completely reversed by warming the reaction mixture to 25°C, 50% inactivation occurs at a concentration of 2.5 mM, NADH protects
Saccharomyces cerevisiae
1.2.1.11
additional information
-
Saccharomyces cerevisiae
1.2.1.11
NADPH-Tris-chloride buffer
promotes a weak inactivation at 0°C, NADH protects
Saccharomyces cerevisiae
1.2.1.11
potassium phosphate
at a concentration of 10 mM promotes 60% inactivation at 0°C in the presence of 10 mM ATP, at a concentration of 100 mM promotes 61% inactivation in the presence of 10 mM ATP and 32% inactivation in the absence of ATP, NADH protects
Saccharomyces cerevisiae
1.2.1.11
Tris salts
100 mM promotes 60.5% inactivation in the presence of 10 mM ATP and 17% inactivation in the absence of ATP
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390198
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390198
Saccharomyces cerevisiae
-
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.11
adenosine 5'-triphosphate, time dependent inactivation at 0°C
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
Adenosine 5'-triphosphate
causes a time-dependent inactivation at a concentration of 3.5 mM, 0°C, pH 6.5 and 2 mM dithiothreitol, inactivation can be completely reversed by warming the reaction mixture to 25°C, 50% inactivation occurs at a concentration of 2.5 mM, NADH protects
Saccharomyces cerevisiae
1.2.1.11
additional information
-
Saccharomyces cerevisiae
1.2.1.11
NADPH-Tris-chloride buffer
promotes a weak inactivation at 0°C, NADH protects
Saccharomyces cerevisiae
1.2.1.11
potassium phosphate
at a concentration of 10 mM promotes 60% inactivation at 0°C in the presence of 10 mM ATP, at a concentration of 100 mM promotes 61% inactivation in the presence of 10 mM ATP and 32% inactivation in the absence of ATP, NADH protects
Saccharomyces cerevisiae
1.2.1.11
Tris salts
100 mM promotes 60.5% inactivation in the presence of 10 mM ATP and 17% inactivation in the absence of ATP
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390198
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390198
Saccharomyces cerevisiae
-