BRENDA - Enzyme Database show

Chemical reactivity at the catalytic sites of aspartic -semialdehyde dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase

Holland, M.J.; Westhead, E.W.; Biochemistry 12, 2276-2281 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
iodoacetamide
at 0.1 mM: 45.4% inactivation in the absence of NADP+, 22% inactivation in the presence of 1 mM NADP+
Saccharomyces cerevisiae
1.2.1.11
iodoacetate
at 1 mM: completely inhibits in the absence or presence of NADP+, at 0.1 mM: 3% inactivation in the absence of NADP+, 50% inactivation in the presence of 1 mM NADP+
Saccharomyces cerevisiae
1.2.1.11
N-ethylmaleimide
only 1 mol per subunit causes complete inactivation, at 0.1 mM: 91% inactivation in the absence of NADP+, 12% inactivation in the presence of 1 mM NADP+
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390197
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390197
Saccharomyces cerevisiae
-
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
iodoacetamide
at 0.1 mM: 45.4% inactivation in the absence of NADP+, 22% inactivation in the presence of 1 mM NADP+
Saccharomyces cerevisiae
1.2.1.11
iodoacetate
at 1 mM: completely inhibits in the absence or presence of NADP+, at 0.1 mM: 3% inactivation in the absence of NADP+, 50% inactivation in the presence of 1 mM NADP+
Saccharomyces cerevisiae
1.2.1.11
N-ethylmaleimide
only 1 mol per subunit causes complete inactivation, at 0.1 mM: 91% inactivation in the absence of NADP+, 12% inactivation in the presence of 1 mM NADP+
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390197
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390197
Saccharomyces cerevisiae
-