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Regulation of the enzymes of lysine biosynthesis in Bacillus sphaericus NCTC 9602 during vegetative growth

Bartlett, A.T.M.; White, P.J.; J. Gen. Microbiol. 132, 3169-3177 (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
L-lysine
slightly represses the enzyme
Lysinibacillus sphaericus
1.4.1.16
D-2,6-diaminopimelate
-
Lysinibacillus sphaericus
1.4.1.16
L-2,6-diaminopimelate
-
Lysinibacillus sphaericus
1.4.1.16
meso-diaminopimelate
-
Lysinibacillus sphaericus
1.17.1.8
1,10-phenanthroline
-
Lysinibacillus sphaericus
1.17.1.8
dipicolinate
-
Lysinibacillus sphaericus
1.17.1.8
additional information
not inhibited by lysine, methionine and threonine
Lysinibacillus sphaericus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
Lysinibacillus sphaericus
-
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
Lysinibacillus sphaericus
r
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
Lysinibacillus sphaericus
highest activity during exponential growth phase
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
Lysinibacillus sphaericus
repressed by lysine
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Lysinibacillus sphaericus
-
-
-
1.4.1.16
Lysinibacillus sphaericus
-
-
-
1.17.1.8
Lysinibacillus sphaericus
-
NCTC 9602
-
Reaction
EC Number
Reaction
Commentary
Organism
1.17.1.8
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+
partly repressed by lysine
Lysinibacillus sphaericus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.04
-
assay system plus lysine or assays system plus lysine, threonine and methionine
Lysinibacillus sphaericus
1.2.1.11
0.043
-
no addition to the assay system or assay system plus methionine
Lysinibacillus sphaericus
1.2.1.11
0.048
-
assay system plus threonine
Lysinibacillus sphaericus
1.4.1.16
0.03
-
reductive amination of 2-amino-6-oxopimelate in crude extracts at pH 7.5 in the presence of 1.67 mM meso-diaminopimelate
Lysinibacillus sphaericus
1.4.1.16
0.25
-
reductive amination of 2-amino-6-oxopimelate in crude extracts at pH 7.5
Lysinibacillus sphaericus
1.4.1.16
0.45
-
oxidative deamination of meso-diaminopimelate in crude extracts at pH 10.5
Lysinibacillus sphaericus
1.17.1.8
0.029
-
during stationary phase
Lysinibacillus sphaericus
1.17.1.8
0.04
-
during late exponential phase
Lysinibacillus sphaericus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390194
Lysinibacillus sphaericus
L-4-aspartyl phosphate + NADPH
-
390194
Lysinibacillus sphaericus
-
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
390194
Lysinibacillus sphaericus
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
390194
Lysinibacillus sphaericus
r
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
390194
Lysinibacillus sphaericus
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
390194
Lysinibacillus sphaericus
r
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
-
390194
Lysinibacillus sphaericus
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
highest activity during exponential growth phase
390194
Lysinibacillus sphaericus
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
repressed by lysine
390194
Lysinibacillus sphaericus
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.1.8
30
-
assay at
Lysinibacillus sphaericus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.8
7.2
-
-
Lysinibacillus sphaericus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.16
NADPH
-
Lysinibacillus sphaericus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.16
NADPH
-
Lysinibacillus sphaericus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
L-lysine
slightly represses the enzyme
Lysinibacillus sphaericus
1.4.1.16
D-2,6-diaminopimelate
-
Lysinibacillus sphaericus
1.4.1.16
L-2,6-diaminopimelate
-
Lysinibacillus sphaericus
1.4.1.16
meso-diaminopimelate
-
Lysinibacillus sphaericus
1.17.1.8
1,10-phenanthroline
-
Lysinibacillus sphaericus
1.17.1.8
dipicolinate
-
Lysinibacillus sphaericus
1.17.1.8
additional information
not inhibited by lysine, methionine and threonine
Lysinibacillus sphaericus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
Lysinibacillus sphaericus
-
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
Lysinibacillus sphaericus
r
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
Lysinibacillus sphaericus
highest activity during exponential growth phase
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
Lysinibacillus sphaericus
repressed by lysine
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.04
-
assay system plus lysine or assays system plus lysine, threonine and methionine
Lysinibacillus sphaericus
1.2.1.11
0.043
-
no addition to the assay system or assay system plus methionine
Lysinibacillus sphaericus
1.2.1.11
0.048
-
assay system plus threonine
Lysinibacillus sphaericus
1.4.1.16
0.03
-
reductive amination of 2-amino-6-oxopimelate in crude extracts at pH 7.5 in the presence of 1.67 mM meso-diaminopimelate
Lysinibacillus sphaericus
1.4.1.16
0.25
-
reductive amination of 2-amino-6-oxopimelate in crude extracts at pH 7.5
Lysinibacillus sphaericus
1.4.1.16
0.45
-
oxidative deamination of meso-diaminopimelate in crude extracts at pH 10.5
Lysinibacillus sphaericus
1.17.1.8
0.029
-
during stationary phase
Lysinibacillus sphaericus
1.17.1.8
0.04
-
during late exponential phase
Lysinibacillus sphaericus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390194
Lysinibacillus sphaericus
L-4-aspartyl phosphate + NADPH
-
390194
Lysinibacillus sphaericus
-
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
390194
Lysinibacillus sphaericus
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
390194
Lysinibacillus sphaericus
r
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
390194
Lysinibacillus sphaericus
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
390194
Lysinibacillus sphaericus
r
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
-
390194
Lysinibacillus sphaericus
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
highest activity during exponential growth phase
390194
Lysinibacillus sphaericus
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
1.17.1.8
2,3-dihydrodipicolinate + NAD(P)H
repressed by lysine
390194
Lysinibacillus sphaericus
2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.1.8
30
-
assay at
Lysinibacillus sphaericus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.8
7.2
-
-
Lysinibacillus sphaericus