BRENDA - Enzyme Database show

Regulation of enzymes of lysine biosynthesis in Corynebacterium glutamicum

Cremer, J.; Treptow, C.; Eggeling, L.; Sahm, H.; J. Gen. Microbiol. 134, 3221-3229 (1988)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
L-isoleucine
inhibits, when added to a final concentration of 10 mM in the assay system produces a decrease of 0.003 units in specific activity
Corynebacterium glutamicum
1.2.1.11
L-leucine
inhibits, when added to a final concentration of 10 mM in the assay system produces a decrease of 0.004 units in specific activity
Corynebacterium glutamicum
1.2.1.11
L-lysine
enzyme assayed in the reverse reaction at pH 10
Corynebacterium glutamicum
1.2.1.11
L-methionine
inhibits, when added to a final concentration of 10 mM in the assay system produces a decrease of 0.004 units in specific activity
Corynebacterium glutamicum
1.2.1.11
L-threonine
enzyme assayed in the reverse reaction at pH 10
Corynebacterium glutamicum
1.2.1.11
additional information
-
Corynebacterium glutamicum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Corynebacterium glutamicum
-
-
-
1.4.1.16
Corynebacterium glutamicum
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.01
-
grown in minimal medium or in minimal medium plus lysine, methionine and threonine
Corynebacterium glutamicum
1.2.1.11
0.012
-
grown in minimal medium plus isoleucine
Corynebacterium glutamicum
1.2.1.11
0.013
-
grown in minimal medium plus lysine or in minimal medium plus threonine
Corynebacterium glutamicum
1.2.1.11
0.015
-
grown in minimal medium plus leucine or in minimal medium plus methionine
Corynebacterium glutamicum
1.2.1.11
0.02
-
grown in complex medium
Corynebacterium glutamicum
1.4.1.16
0.15
-
activity in crude extracts of cells grown on complex medium
Corynebacterium glutamicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390193
Corynebacterium glutamicum
L-4-aspartyl phosphate + NADPH
-
390193
Corynebacterium glutamicum
-
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
390193
Corynebacterium glutamicum
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
390193
Corynebacterium glutamicum
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Corynebacterium glutamicum
1.4.1.16
NADPH
-
Corynebacterium glutamicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Corynebacterium glutamicum
1.4.1.16
NADPH
-
Corynebacterium glutamicum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
L-isoleucine
inhibits, when added to a final concentration of 10 mM in the assay system produces a decrease of 0.003 units in specific activity
Corynebacterium glutamicum
1.2.1.11
L-leucine
inhibits, when added to a final concentration of 10 mM in the assay system produces a decrease of 0.004 units in specific activity
Corynebacterium glutamicum
1.2.1.11
L-lysine
enzyme assayed in the reverse reaction at pH 10
Corynebacterium glutamicum
1.2.1.11
L-methionine
inhibits, when added to a final concentration of 10 mM in the assay system produces a decrease of 0.004 units in specific activity
Corynebacterium glutamicum
1.2.1.11
L-threonine
enzyme assayed in the reverse reaction at pH 10
Corynebacterium glutamicum
1.2.1.11
additional information
-
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.01
-
grown in minimal medium or in minimal medium plus lysine, methionine and threonine
Corynebacterium glutamicum
1.2.1.11
0.012
-
grown in minimal medium plus isoleucine
Corynebacterium glutamicum
1.2.1.11
0.013
-
grown in minimal medium plus lysine or in minimal medium plus threonine
Corynebacterium glutamicum
1.2.1.11
0.015
-
grown in minimal medium plus leucine or in minimal medium plus methionine
Corynebacterium glutamicum
1.2.1.11
0.02
-
grown in complex medium
Corynebacterium glutamicum
1.4.1.16
0.15
-
activity in crude extracts of cells grown on complex medium
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390193
Corynebacterium glutamicum
L-4-aspartyl phosphate + NADPH
-
390193
Corynebacterium glutamicum
-
1.4.1.16
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
390193
Corynebacterium glutamicum
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
390193
Corynebacterium glutamicum
r