BRENDA - Enzyme Database show

Cloning of the aspartate-beta-semialdehyde dehydrogenase structural gene from Escherichia coli K12

Preiss, J.; Mazelis, M.; Greenberg, E.; Curr. Microbiol. 7, 263-268 (1982)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.2
-
L-aspartate 4-semialdehyde
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
40000
-
2 * 40000
Escherichia coli
1.2.1.11
77000
-
sucrose density gradient centrifugation
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Escherichia coli
-
K-12
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
using heat treatment, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-200
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.002
-
K12 6201 strain, enriched media plus diaminopimelic acid
Escherichia coli
1.2.1.11
0.12
-
B AC70R1 strain, enriched media
Escherichia coli
1.2.1.11
0.4
-
K12 23631 strain, minimal media plus N-formyllysine
Escherichia coli
1.2.1.11
0.94
-
B AC70R1 strain, minimal media
Escherichia coli
1.2.1.11
1.8
-
K12 6201/PoP126 strain, enriched media plus ampicillin
Escherichia coli
1.2.1.11
2.7
-
B AC70R1/PoP12 strain, enriched media plus tetracycline
Escherichia coli
1.2.1.11
5.3
-
B AC70R1/PoP12 strain, minimal media plus tetracycline
Escherichia coli
1.2.1.11
26
-
K12 23631/pOP12 strain, minimal media plus N-formyllysine and tetracycline
Escherichia coli
1.2.1.11
145
-
K12 23631/pOP12, after three times purification of the enzyme
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390191
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390191
Escherichia coli
-
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
dimer
2 * 40000
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.2
-
L-aspartate 4-semialdehyde
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
40000
-
2 * 40000
Escherichia coli
1.2.1.11
77000
-
sucrose density gradient centrifugation
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
using heat treatment, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-200
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
0.002
-
K12 6201 strain, enriched media plus diaminopimelic acid
Escherichia coli
1.2.1.11
0.12
-
B AC70R1 strain, enriched media
Escherichia coli
1.2.1.11
0.4
-
K12 23631 strain, minimal media plus N-formyllysine
Escherichia coli
1.2.1.11
0.94
-
B AC70R1 strain, minimal media
Escherichia coli
1.2.1.11
1.8
-
K12 6201/PoP126 strain, enriched media plus ampicillin
Escherichia coli
1.2.1.11
2.7
-
B AC70R1/PoP12 strain, enriched media plus tetracycline
Escherichia coli
1.2.1.11
5.3
-
B AC70R1/PoP12 strain, minimal media plus tetracycline
Escherichia coli
1.2.1.11
26
-
K12 23631/pOP12 strain, minimal media plus N-formyllysine and tetracycline
Escherichia coli
1.2.1.11
145
-
K12 23631/pOP12, after three times purification of the enzyme
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390191
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390191
Escherichia coli
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
dimer
2 * 40000
Escherichia coli