BRENDA - Enzyme Database show

Semi-aldehyde aspartic dehydrogenase of Saccharomyces cerevisiae: properties and regulation

Surdin, Y.; Eur. J. Biochem. 2, 341-348 (1967)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.11
H2CO3
enhances activity 4 or 5 times in both directions
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
cysteine
in the reverse reaction
Saccharomyces cerevisiae
1.2.1.11
homocysteine
in the reverse reaction
Saccharomyces cerevisiae
1.2.1.11
L-methionine
-
Saccharomyces cerevisiae
1.2.1.11
L-threonine
-
Saccharomyces cerevisiae
1.2.1.11
additional information
no feed-back inhibition by threonine or methionine
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
1.4
-
phosphate
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
140000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Saccharomyces cerevisiae
synthesis of threonine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Saccharomyces cerevisiae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Saccharomyces cerevisiae
synthesis of methionine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Saccharomyces cerevisiae
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
using ammonium sulfate precipitation and column chromatography on Sephadex G-200, DEAE-cellulose and hydroxylapatite
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
synthesis of threonine
390187
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390187
Saccharomyces cerevisiae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
synthesis of methionine
390187
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390187
Saccharomyces cerevisiae
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390187
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390187
Saccharomyces cerevisiae
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
8.5
-
in both directions
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Saccharomyces cerevisiae
1.2.1.11
NADPH
-
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
10
-
homocysteine
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.11
H2CO3
enhances activity 4 or 5 times in both directions
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Saccharomyces cerevisiae
1.2.1.11
NADPH
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
cysteine
in the reverse reaction
Saccharomyces cerevisiae
1.2.1.11
homocysteine
in the reverse reaction
Saccharomyces cerevisiae
1.2.1.11
L-methionine
-
Saccharomyces cerevisiae
1.2.1.11
L-threonine
-
Saccharomyces cerevisiae
1.2.1.11
additional information
no feed-back inhibition by threonine or methionine
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
10
-
homocysteine
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
1.4
-
phosphate
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
140000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Saccharomyces cerevisiae
synthesis of threonine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Saccharomyces cerevisiae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
Saccharomyces cerevisiae
synthesis of methionine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Saccharomyces cerevisiae
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
using ammonium sulfate precipitation and column chromatography on Sephadex G-200, DEAE-cellulose and hydroxylapatite
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
synthesis of threonine
390187
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390187
Saccharomyces cerevisiae
-
1.2.1.11
L-4-aspartyl phosphate + NADPH
synthesis of methionine
390187
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390187
Saccharomyces cerevisiae
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390187
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390187
Saccharomyces cerevisiae
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
8.5
-
in both directions
Saccharomyces cerevisiae