BRENDA - Enzyme Database show

Studies of a homoserineless bradytroph of Neurospora crassa: demonstration of an altered aspartate beta-semialdehyde dehydrogenase

Jenkins, M.B.; Garner, H.R.; Biochim. Biophys. Acta 141, 287-295 (1967)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.02
-
NADPH
mutant
Neurospora crassa
1.2.1.11
0.025
-
NADPH
wild type
Neurospora crassa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Neurospora crassa
-
wild type strain G165-3A and mutant strain G184-1A
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
-
Neurospora crassa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390186
Neurospora crassa
L-4-aspartyl phosphate + NADPH
-
390186
Neurospora crassa
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
19
-
mutant
Neurospora crassa
1.2.1.11
28
-
above 28°C, wild type
Neurospora crassa
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.11
50
-
3 min, 50% loss of activity, mutant, 5 min, no loss of activity, wild type
Neurospora crassa
1.2.1.11
65
-
30 min, wild type, stable
Neurospora crassa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
7.4
7.8
mutant
Neurospora crassa
1.2.1.11
8
-
wild type
Neurospora crassa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.11
6.5
9
pH 6.5: about 45% of activity maximum, mutant, about 8% of activity maximum, wild type, pH 9.0: about 55% of activity maximum, mutant, about 30% of activity maximum, wild type
Neurospora crassa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.02
-
NADPH
mutant
Neurospora crassa
1.2.1.11
0.025
-
NADPH
wild type
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
additional information
-
-
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390186
Neurospora crassa
L-4-aspartyl phosphate + NADPH
-
390186
Neurospora crassa
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
19
-
mutant
Neurospora crassa
1.2.1.11
28
-
above 28°C, wild type
Neurospora crassa
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.11
50
-
3 min, 50% loss of activity, mutant, 5 min, no loss of activity, wild type
Neurospora crassa
1.2.1.11
65
-
30 min, wild type, stable
Neurospora crassa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
7.4
7.8
mutant
Neurospora crassa
1.2.1.11
8
-
wild type
Neurospora crassa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.11
6.5
9
pH 6.5: about 45% of activity maximum, mutant, about 8% of activity maximum, wild type, pH 9.0: about 55% of activity maximum, mutant, about 30% of activity maximum, wild type
Neurospora crassa