BRENDA - Enzyme Database show

Affinity labeling of the Escherichia coli aspartate-beta-semialdehyde dehydrogenase with an alkylating coenzyme analogue. Half-site reactivity and competition with the substrate alkylating analogue

Bielmann, J.F.; Eid, P.; Hirth, C.; Eur. J. Biochem. 104, 65-69 (1980)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
3-Chloroacetylpyridine-adenine dinucleotide phosphate
NADP+ and NADPH protect
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390184
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390184
Escherichia coli
-
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
0.04
-
3-Chloroacetylpyridine-adenine dinucleotide phosphate
competitive inhibitor with respect to NADP+
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
3-Chloroacetylpyridine-adenine dinucleotide phosphate
NADP+ and NADPH protect
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.11
0.04
-
3-Chloroacetylpyridine-adenine dinucleotide phosphate
competitive inhibitor with respect to NADP+
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390184
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390184
Escherichia coli
-