BRENDA - Enzyme Database show

Aspartate-beta-semialdehyde dehydrogenase from Escherichia coli. Purification and general properties

Biellmann, J.F.; Eid, P.; Hirth, C.; Jörnvall, H.; Eur. J. Biochem. 104, 53-58 (1980)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
38000
-
2 * 38000, SDS-PAGE
Escherichia coli
1.2.1.11
77500
-
ultracentrifugation
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Escherichia coli
enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Escherichia coli
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Escherichia coli
-
mutant derepressed for aspartate-beta-semialdehyde dehydrogenase biosynthesis
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, hydroxylapatite, ultrogel ACA-44 and Sepharose C3
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
100
110
-
Escherichia coli
1.2.1.11
105
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.11
-20°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, 50% glycerol, 2 months
Escherichia coli
1.2.1.11
4°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, several days
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine
390183
Escherichia coli
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390183
Escherichia coli
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390183
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390183
Escherichia coli
-
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
dimer
2 * 38000, SDS-PAGE
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Escherichia coli
1.2.1.11
NADPH
L-aspartate 4-semialdehyde dehydrogenase transfers the pro-S hydrogen from NADPH
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Escherichia coli
1.2.1.11
NADPH
L-aspartate 4-semialdehyde dehydrogenase transfers the pro-S hydrogen from NADPH
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
38000
-
2 * 38000, SDS-PAGE
Escherichia coli
1.2.1.11
77500
-
ultracentrifugation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Escherichia coli
enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Escherichia coli
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, hydroxylapatite, ultrogel ACA-44 and Sepharose C3
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
100
110
-
Escherichia coli
1.2.1.11
105
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.11
-20°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, 50% glycerol, 2 months
Escherichia coli
1.2.1.11
4°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, several days
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine
390183
Escherichia coli
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390183
Escherichia coli
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390183
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390183
Escherichia coli
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
dimer
2 * 38000, SDS-PAGE
Escherichia coli