BRENDA - Enzyme Database show

Purification and characterization of aspartic -semialdehyde dehydrogenase from yeast and purification of an isozyme of glyceraldehyde-3-phosphate dehydrogenase

Holland, M.J.; Westhead, E.W.; Biochemistry 12, 2264-2270 (1973)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.036
-
NADP+
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
41000
-
4 * 41000, SDS-PAGE
Saccharomyces cerevisiae
1.2.1.11
156000
-
sedimentation equilibrium, meniscus depletion technique
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Saccharomyces cerevisiae
synthesis of threonine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Saccharomyces cerevisiae
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
using protamine sulfate treatment, ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-50 column, Bio-Gel hydroxylapatite phosphate column, Bio-Gel hydroxylapatite sulfate column, acid treatment and column chromatography on Sephadex G-200
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
73
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
synthesis of threonine
390182
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390182
Saccharomyces cerevisiae
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390182
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390182
Saccharomyces cerevisiae
-
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
tetramer
4 * 41000, SDS-PAGE
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADPH
-
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADPH
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.036
-
NADP+
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
41000
-
4 * 41000, SDS-PAGE
Saccharomyces cerevisiae
1.2.1.11
156000
-
sedimentation equilibrium, meniscus depletion technique
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
Saccharomyces cerevisiae
synthesis of threonine
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Saccharomyces cerevisiae
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
using protamine sulfate treatment, ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-50 column, Bio-Gel hydroxylapatite phosphate column, Bio-Gel hydroxylapatite sulfate column, acid treatment and column chromatography on Sephadex G-200
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
73
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH
synthesis of threonine
390182
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390182
Saccharomyces cerevisiae
-
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390182
Saccharomyces cerevisiae
L-4-aspartyl phosphate + NADPH
-
390182
Saccharomyces cerevisiae
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
tetramer
4 * 41000, SDS-PAGE
Saccharomyces cerevisiae