BRENDA - Enzyme Database show

Aspartic semialdehyde dehydrogenase (Escherichia coli K12)

Hegeman, G.D.; Cohen, G.N.; Morgan, R.; Methods Enzymol. 17A, 708-713 (1970)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.2.1.11
lyophilization of purified or partially purified enzyme, slight loss of activity
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
aromatic aldehydes
e.g.: benzaldehyde, weak
Escherichia coli
1.2.1.11
Borate
-
Escherichia coli
1.2.1.11
formaldehyde
-
Escherichia coli
1.2.1.11
Glutaraldehyde
-
Escherichia coli
1.2.1.11
iodoacetate
-
Escherichia coli
1.2.1.11
K+
-
Escherichia coli
1.2.1.11
additional information
not: chelating agents
Escherichia coli
1.2.1.11
N-ethylmaleimide
-
Escherichia coli
1.2.1.11
Na+
-
Escherichia coli
1.2.1.11
NH4+
-
Escherichia coli
1.2.1.11
p-hydroxymercuribenzoate
-
Escherichia coli
1.2.1.11
Tris salts
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.09
-
NADP+
-
Escherichia coli
1.2.1.11
0.2
-
L-aspartate 4-semialdehyde
-
Escherichia coli
1.2.1.11
6
-
HAsO42-
-
Escherichia coli
1.2.1.11
9
-
phosphate
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
70000
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Escherichia coli
-
K-12
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
K-12, using ammonium sulfate precipitation and column chromatography on Sephadex G-200 and DEAE-Sephadex A-50
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
120
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.11
frozen or on ice, after dialysis, 1 month stable
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390180
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390180
Escherichia coli
-
1.2.1.11
additional information
maximum velocity with HAsO42- is 0.4 times that with HPO42-
390180
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
25
-
assay at
Escherichia coli
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.11
50
-
20 min, crude extract, 20% loss of activity
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
-
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
additional information
NAD+: 0.1% or less the rate of reaction with NADP+
Escherichia coli
1.2.1.11
NADP+
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
additional information
NAD+: 0.1% or less the rate of reaction with NADP+
Escherichia coli
1.2.1.11
NADP+
-
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.11
lyophilization of purified or partially purified enzyme, slight loss of activity
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
aromatic aldehydes
e.g.: benzaldehyde, weak
Escherichia coli
1.2.1.11
Borate
-
Escherichia coli
1.2.1.11
formaldehyde
-
Escherichia coli
1.2.1.11
Glutaraldehyde
-
Escherichia coli
1.2.1.11
iodoacetate
-
Escherichia coli
1.2.1.11
K+
-
Escherichia coli
1.2.1.11
additional information
not: chelating agents
Escherichia coli
1.2.1.11
N-ethylmaleimide
-
Escherichia coli
1.2.1.11
Na+
-
Escherichia coli
1.2.1.11
NH4+
-
Escherichia coli
1.2.1.11
p-hydroxymercuribenzoate
-
Escherichia coli
1.2.1.11
Tris salts
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.09
-
NADP+
-
Escherichia coli
1.2.1.11
0.2
-
L-aspartate 4-semialdehyde
-
Escherichia coli
1.2.1.11
6
-
HAsO42-
-
Escherichia coli
1.2.1.11
9
-
phosphate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
70000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
K-12, using ammonium sulfate precipitation and column chromatography on Sephadex G-200 and DEAE-Sephadex A-50
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.11
120
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.11
frozen or on ice, after dialysis, 1 month stable
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
390180
Escherichia coli
L-4-aspartyl phosphate + NADPH
-
390180
Escherichia coli
-
1.2.1.11
additional information
maximum velocity with HAsO42- is 0.4 times that with HPO42-
390180
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.11
25
-
assay at
Escherichia coli
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.11
50
-
20 min, crude extract, 20% loss of activity
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.11
9
-
-
Escherichia coli