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Literature summary extracted from

  • Kazahaya, T.; Kawai, K.; Yashima, S.; Sasaki, Y.
    Aerobic dissimilation of glucose by heterolactic bacteria. III. Aldehyde dehydrogenase and alcohol dehydrogenase of Leuconostoc mesenteroides (1972), J. Gen. Appl. Microbiol., 18, 43-55.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.10 GSH 7fold activation Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.10 ADP 1 mM 42% inhibition, competitive to NAD+ Leuconostoc mesenteroides
1.2.1.10 AgNO3 1 mM 20% inhibition Leuconostoc mesenteroides
1.2.1.10 AMP 1 mM 48% inhibition, competitive to NAD+ Leuconostoc mesenteroides
1.2.1.10 ATP 1 mM 38% inhibition, competitive to NAD+ Leuconostoc mesenteroides
1.2.1.10 CuSO4 1 mM 49% inhibition Leuconostoc mesenteroides
1.2.1.10 HgCl2 1 mM 31% inhibition Leuconostoc mesenteroides
1.2.1.10 additional information low ionic strength buffers, 0.2 M boric acid/Na2CO3, 0.2 M glycine/NaOH, 0.2 M NH4Cl/NH4OH, pH 8.0, not inhibitory at 1 mM: K+, Na+, Li+, Mg2+, Mn2+, Zn2 +, Ca2+, Fe2+, Fe3+, EDTA, p-chloromercuribenzoate, iodoacetate, N3-, dipyridyl, lactic acid, acetic acid, ethanol, acetylphosphate, pyruvic acid, adenine, adenosine, guanine, guanosine, GMP, GDP, not inhibitory at 0.1 mM: NADP+, NADPH Leuconostoc mesenteroides
1.2.1.10 Na2HAsO4 1 mM 100% inhibition, 0.1 mM 32% inhibition Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.10 0.009
-
 CoA
-
 Leuconostoc mesenteroides
1.2.1.10 0.17
-
 NAD+
-
 Leuconostoc mesenteroides
1.2.1.10 3.7
-
 acetaldehyde
-
 Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+ Leuconostoc mesenteroides ethanol production in aerobic glucose dissimilation acetyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Leuconostoc mesenteroides
-
 IFO 3426
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.10 partial Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.10 0.87
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Leuconostoc mesenteroides acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+ ethanol production in aerobic glucose dissimilation Leuconostoc mesenteroides acetyl-CoA + NADH
-
 r
1.2.1.10 isobutyraldehyde + CoA + NAD+
-
 Leuconostoc mesenteroides isobutyryl-CoA + NADH
-
 ?
1.2.1.10 additional information not: benzaldehyde Leuconostoc mesenteroides ?
-
 ?
1.2.1.10 n-butyraldehyde + CoA + NAD+
-
 Leuconostoc mesenteroides n-butyryl-CoA + NADH
-
 r
1.2.1.10 propanal + CoA + NAD+
-
 Leuconostoc mesenteroides propionyl-CoA + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.10 30
-
-
 Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.10 40
-
 15 min, 30% activity left Leuconostoc mesenteroides
1.2.1.10 45
-
 15 min, inactivation Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.10 8
-
-
 Leuconostoc mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.10 CoA
-
 Leuconostoc mesenteroides
1.2.1.10 NAD+ no reaction with NADP+ Leuconostoc mesenteroides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.10 0.75
-
 AMP
-
 Leuconostoc mesenteroides
1.2.1.10 2.5
-
 ADP
-
 Leuconostoc mesenteroides
1.2.1.10 8.6
-
 ATP
-
 Leuconostoc mesenteroides