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Literature summary extracted from

  • Hosoi, N.; Ozaki, C.; Kitamoto, Y.; Ichikawa, Y.
    Purification and properties of aldehyde dehydrogenase (acylating) from propionibacterium freudenreichii (1979), J. Ferment. Technol., 57, 418-427.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.10 2-mercaptoethanol
-
 Propionibacterium freudenreichii
1.2.1.10 sulfhydryl compound requirement for reduced form Propionibacterium freudenreichii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.10 0.04
-
 CoA
-
 Propionibacterium freudenreichii
1.2.1.10 0.43
-
 NAD+
-
 Propionibacterium freudenreichii
1.2.1.10 1.5
-
 Valeraldehyde
-
 Propionibacterium freudenreichii
1.2.1.10 6.9
-
 propanal
-
 Propionibacterium freudenreichii
1.2.1.10 100
-
 acetaldehyde
-
 Propionibacterium freudenreichii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.10 48000
-
 4 * 48000, SDS-PAGE Propionibacterium freudenreichii
1.2.1.10 188000
-
 gel filtration Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+ Propionibacterium freudenreichii
-
 acetyl-CoA + NADH
-
 ?
1.2.1.10 propanal + CoA + NAD+ Propionibacterium freudenreichii fermentation of 1,2-propanediol propionyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Propionibacterium freudenreichii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.10
-
 Propionibacterium freudenreichii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.10 83.7
-
-
 Propionibacterium freudenreichii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii acetyl-CoA + NADH
-
 ?
1.2.1.10 glutaraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii glutaryl-CoA + NADH
-
 ?
1.2.1.10 glyoxal + CoA + NAD+
-
 Propionibacterium freudenreichii glyoxyl-CoA + NADH
-
 ?
1.2.1.10 heptylaldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii heptanoyl-CoA + NADH
-
 ?
1.2.1.10 hexylaldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii hexanoyl-CoA + NADH
-
 ?
1.2.1.10 isobutyraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii isobutyryl-CoA + NADH
-
 ?
1.2.1.10 additional information not: formaldehyde, octylaldehyde, benzaldehyde, D,L-glyceraldehyde, glycolaldehyde Propionibacterium freudenreichii ?
-
 ?
1.2.1.10 n-butyraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii n-butyryl-CoA + NADH
-
 r
1.2.1.10 propanal + CoA + NAD+ fermentation of 1,2-propanediol Propionibacterium freudenreichii propionyl-CoA + NADH
-
 ?
1.2.1.10 propanal + CoA + NAD+ fermentation of 1,2-propanediol Propionibacterium freudenreichii propionyl-CoA + NADH
-
 r
1.2.1.10 valeraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii valeryl-CoA + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.10 tetramer 4 * 48000, SDS-PAGE Propionibacterium freudenreichii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.10 45
-
-
 Propionibacterium freudenreichii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.10 20 60 55% of maximal activity at 20°C, 60% at 60°C Propionibacterium freudenreichii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.10 70
-
 3 min, inactivation above Propionibacterium freudenreichii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.10 7 9.5 30% of maximal activity at pH 7.0, 100% at pH 9.5 Propionibacterium freudenreichii
1.2.1.10 8.2 9.5
-
 Propionibacterium freudenreichii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.10 CoA
-
 Propionibacterium freudenreichii
1.2.1.10 NAD+ no reaction with NADP+ Propionibacterium freudenreichii