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Literature summary extracted from

  • Shone, C.C.; Fromm, H.J.
    Steady-state and pre-steady-state kinetics of coenzyme A linked aldehyde dehydrogenase from Escherichia coli (1981), Biochemistry, 20, 7494-7501.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.10 2-mercaptoethanol reduces lag phase before attainment of steady state rate in conjunction with NAD+ Escherichia coli
1.2.1.10 3-pyridinecarboxaldehyde adenine dinucleotide NAD+ analogue, activates by binding strongly to activator site, binds weakly to catalytic site Escherichia coli
1.2.1.10 NAD+ reduces lag phase before attainment of steady state rate in conjunction with 2-mercaptoethanol Escherichia coli

General Stability

EC Number General Stability Organism
1.2.1.10 labile during purification, stabilization by 2-hydroxyethyldisulfide Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.10 ADP-ribose
-
 Escherichia coli
1.2.1.10 AMP
-
 Escherichia coli
1.2.1.10 benzaldehyde
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.10 0.007
-
 acetyl-CoA
-
 Escherichia coli
1.2.1.10 0.008
-
 CoA
-
 Escherichia coli
1.2.1.10 0.025
-
 NADH
-
 Escherichia coli
1.2.1.10 0.08
-
 NAD+
-
 Escherichia coli
1.2.1.10 10
-
 acetaldehyde
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+ Escherichia coli during glucose fermentation acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+ Escherichia coli B / ATCC 11303 during glucose fermentation acetyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Escherichia coli
-
-
-
1.2.1.10 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.10 partial Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.10 acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH + H+ bi-uni-uni-uni-ping-pong mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.10 14.1
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Escherichia coli acetyl-CoA + NADH
-
 ?
1.2.1.10 acetaldehyde + CoA + NAD+ during glucose fermentation Escherichia coli acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Escherichia coli B / ATCC 11303 acetyl-CoA + NADH
-
 ?
1.2.1.10 acetaldehyde + CoA + NAD+ during glucose fermentation Escherichia coli B / ATCC 11303 acetyl-CoA + NADH
-
 r
1.2.1.10 additional information not benzaldehyde Escherichia coli ?
-
 ?
1.2.1.10 additional information not benzaldehyde Escherichia coli B / ATCC 11303 ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.10 CoA
-
 Escherichia coli
1.2.1.10 NAD+
-
 Escherichia coli