Literature summary extracted from
Abreu, I.A.; Saraiva, L.M.; Carita, J.; Huber, H.; Stetter, K.O.; Cabelli, D.; Teixeira, M.
Oxygen detoxification in the strict anaerobic archaeon Archaeoglobus fulgidus: superoxide scavenging by Neelaredoxin (2000), Mol. Microbiol., 38, 322-334.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.15.1.2 |
expression in Escherichia coli |
Archaeoglobus fulgidus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.15.1.2 |
Fe2+/Fe3+ |
enzyme contains 1 iron atom/monomer |
Archaeoglobus fulgidus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.15.1.2 |
Archaeoglobus fulgidus |
- |
strict anaerobic archaeon, the oxidized enzyme exhibits a characteristic blue color that prompted the name neelaredoxin, derived from the sanskrit word for blue |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.15.1.2 |
native and recombinant enzyme |
Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.15.1.2 |
additional information |
- |
60.0 U/mg, 1 U is the amount of enzyme necessary to inhibit 50% of the reduction of cytochrome c by the xanthine/xanthine oxidase system, superoxid dismutase activity of native enzyme |
Archaeoglobus fulgidus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.15.1.2 |
reduced acceptor + superoxide |
enzyme is able to both reduce and dismutate superoxide |
Archaeoglobus fulgidus |
acceptor + H2O2 + O2 |
- |
? |
|
1.15.1.2 |
reduced acceptor + superoxide + H+ |
enzyme can be fully reduced upon addition of NADH or NADPH under anaerobic conditions |
Archaeoglobus fulgidus |
acceptor + H2O2 |
- |
? |
|