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Literature summary extracted from

  • Abreu, I.A.; Saraiva, L.M.; Carita, J.; Huber, H.; Stetter, K.O.; Cabelli, D.; Teixeira, M.
    Oxygen detoxification in the strict anaerobic archaeon Archaeoglobus fulgidus: superoxide scavenging by Neelaredoxin (2000), Mol. Microbiol., 38, 322-334.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.2 expression in Escherichia coli Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.2 Fe2+/Fe3+ enzyme contains 1 iron atom/monomer Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.2 Archaeoglobus fulgidus
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strict anaerobic archaeon, the oxidized enzyme exhibits a characteristic blue color that prompted the name neelaredoxin, derived from the sanskrit word for blue
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.2 native and recombinant enzyme Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.2 additional information
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60.0 U/mg, 1 U is the amount of enzyme necessary to inhibit 50% of the reduction of cytochrome c by the xanthine/xanthine oxidase system, superoxid dismutase activity of native enzyme Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.2 reduced acceptor + superoxide enzyme is able to both reduce and dismutate superoxide Archaeoglobus fulgidus acceptor + H2O2 + O2
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1.15.1.2 reduced acceptor + superoxide + H+ enzyme can be fully reduced upon addition of NADH or NADPH under anaerobic conditions Archaeoglobus fulgidus acceptor + H2O2
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