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Literature summary extracted from
- C21 steroid side chain cleavage enzyme from porcine adrenal microsomes. Purification and characterization of the 17alpha-hydroxylase/C17,20-lyase cytochrome P-450 (1984), J. Biol. Chem., 259, 3971-3976.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | sodium cholate | - |
Sus scrofa |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.19 | 0.0008 | - |
pregnenolone | adrenal gland | Sus scrofa | |
| 1.14.14.19 | 0.0009 | - |
17alpha-hydroxypregnenolone | adrenal gland | Sus scrofa | |
| 1.14.14.19 | 0.0018 | - |
progesterone | adrenal gland | Sus scrofa | |
| 1.14.14.19 | 0.0025 | - |
17alpha-hydroxyprogesterone | adrenal gland | Sus scrofa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.19 | microsome | - |
Sus scrofa | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | Fe3+ | heme content more than 0.8 nM/nM protein | Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.19 | 54000 | - |
SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.19 | pregnenolone + AH2 + O2 | Sus scrofa | - |
17alpha-hydroxypregnenolone + A + H2O | product eliminates at C20,21 acetate to yield dehydroepiandrosterone | ? | |
| 1.14.14.19 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Sus scrofa | - |
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.19 | Sus scrofa | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.19 | adrenal gland | little lyase activity | Sus scrofa | - |
| 1.14.14.19 | testis | greater lyase than hydroxylase activity | Sus scrofa | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.19 | -70°C, 0.2% Emulgen 913 v/v and 20% glycerol v/v, 6 months, no detectable loss of activity | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.19 | pregnenolone + AH2 + O2 | - |
Sus scrofa | 17alpha-hydroxypregnenolone + A + H2O | product eliminates at C20,21 acetate to yield dehydroepiandrosterone | ? | |
| 1.14.14.19 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sus scrofa | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | NADPH | - |
Sus scrofa | |
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