Literature summary extracted from

Sakaki, T.; Shibata, M.; Yabusaki, Y.; Murakami, H.; Ohkawa, H.
Genetically engineered P450 monooxygenases: construction of bovine P450c17/yeast reductase fused enzymes (1990), DNA Cell Biol., 9, 27-36.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.14.19	expression in Saccharomyces cerevisiae strain AH22, fused with yeast reductase, greater 17alpha-hydroxylase activity	Bos taurus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.14.19	microsome	-	Bos taurus	-	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.14.14.19	progesterone + [reduced NADPH-hemoprotein reductase] + O2	Bos taurus	-	17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O	product eliminates at C20,21 acetate to yield androstenedione	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.19	Bos taurus	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.19	progesterone + [reduced NADPH-hemoprotein reductase] + O2	-	Bos taurus	17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O	product eliminates at C20,21 acetate to yield androstenedione	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.14.14.19	1.55	-	progesterone	17alpha-hydroxylase activity	Bos taurus

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Release 2023.1 (January 2023)