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Literature summary extracted from

  • Park, S.H.; Lee, C.G.; Namkung, R.; Kim, C.G.; Park, S.D.
    Active DNA topoisomerase II with minimum molecular mass from regenerating rat liver (1994), Biochem. Mol. Biol. Int., 32, 641-650.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 expression in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 Nalidixic acid no inhibition Rattus norvegicus
5.6.2.2 novobiocin 1 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.2 173000
-
 bands of MW 55000 and MW 70000 detected by SDS-PAGE represent proteolytic products of the 173000 MW form, however these products retain the enzyme activities. The topoisomerase II consists of different functional domains, the whole enzyme is not required for its activity Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 liver regenerating Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 network of DNA rings + ATP + H2O decatenation Rattus norvegicus monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation Rattus norvegicus catenated DNA networks + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation Rattus norvegicus relaxed DNA + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.2 monomer 1 * 173000, bands of MW 55000 and MW 70000 detected by SDS-PAGE represent proteolytic products of the 173000 MW form, however these products retain the enzyme activities. The topoisomerase II consists of different functional domains, the whole enzyme is not required for its activity Rattus norvegicus