Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Haudenschild, C.; Schalk, M.; Karp, F.; Croteau, R.
    Functional expression of regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha spp.) in Escherichia coli and Saccharomyces cerevisiae (2000), Arch. Biochem. Biophys., 379, 127-136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.51 expression in Escherichia coli and in Saccharomyces cerevisiae Mentha spicata
1.14.14.99 expression in Escherichia coli and Saccharomyces cerevisiae Mentha x piperita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.51 57000
-
 SDS-PAGE, recombinant protein expressed in Saccharomyces cerevisiae Mentha spicata

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.51 Mentha spicata
-
 spearmint
-
1.14.14.99 Mentha x piperita
-
 peppermint
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.51 protein expressed in Escherichia coli Mentha spicata
1.14.14.51 protein expressed in Saccharomyces cerevisiae Mentha spicata
1.14.14.99 recombinant protein from Escherichia coli Mentha x piperita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.51 (-)-(4S)-limonene + NADPH + O2
-
 Mentha spicata (-)-(4R,6S)-trans-carveol + NADP+ + H2O
-
 ?
1.14.14.99 (-)-(4S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 highly specific, regiospecific and stereospecific reaction Mentha x piperita (-)-trans-(3S,4R)-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?