EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.2 | 3-hydroxybenzoate | 2 mM, 26% inhibition | Moraxella sp. | |
1.14.13.2 | 4-hydroxy-3-nitrobenzoic acid | 2 mM, 23% inhibition | Moraxella sp. | |
1.14.13.2 | 4-hydroxybenzaldehyde | 2 mM, 27% inhibition | Moraxella sp. | |
1.14.13.2 | 4-hydroxybenzoate | above 0.1 mM | Moraxella sp. | |
1.14.13.2 | 4-hydroxyphenylacetic acid | 2 mM, 16% inhibition | Moraxella sp. | |
1.14.13.2 | 4-nitrophenol | 2 mM, 39% inhibition | Moraxella sp. | |
1.14.13.2 | benzoate | 2 mM, 48% inhibition | Moraxella sp. | |
1.14.13.2 | coumaric acid | 2 mM, 30% inhibition | Moraxella sp. | |
1.14.13.2 | Hg2+ | 0.1 mM HgCl2, complete inhibition | Moraxella sp. | |
1.14.13.2 | PCMB | 0.015 mM, more than 70% inhibition, partially restored by addition of 0.14 M 2-mercaptoethanol | Moraxella sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | 0.0095 | - |
4-hydroxybenzoate | reaction with NADH | Moraxella sp. | |
1.14.13.2 | 0.037 | - |
NADH | - |
Moraxella sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.2 | 45000 | - |
2 * 45000, SDS-PAGE | Moraxella sp. |
1.14.13.2 | 85000 | - |
gel filtration | Moraxella sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | Moraxella sp. | degradation of 4-hydroxybenzoate | ? | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | Moraxella sp. GU2 | degradation of 4-hydroxybenzoate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.2 | Moraxella sp. | - |
- |
- |
1.14.13.2 | Moraxella sp. GU2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.2 | - |
Moraxella sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.2 | 10.2 | - |
- |
Moraxella sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.2 | -70°C, 5 mM potassium phosphate, pH 7.5, 5% glycerol, stable for several months | Moraxella sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 4-hydroxybenzoate + NADH + O2 | - |
Moraxella sp. | protocatechuate + NAD+ + H2O | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADH + O2 | - |
Moraxella sp. GU2 | protocatechuate + NAD+ + H2O | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | - |
Moraxella sp. | protocatechuate + NADP+ + H2O | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | - |
Moraxella sp. GU2 | protocatechuate + NADP+ + H2O | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | degradation of 4-hydroxybenzoate | Moraxella sp. | ? | - |
? | |
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | degradation of 4-hydroxybenzoate | Moraxella sp. GU2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.2 | dimer | 2 * 45000, SDS-PAGE | Moraxella sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.2 | 52 | - |
pH 7.5, 20 mM potassium phosphate buffer, 20 min, stable | Moraxella sp. |
1.14.13.2 | 60 | - |
in presence of 1 mM 4-hydroxybenzoate, 40% loss of activity after 20 min | Moraxella sp. |
1.14.13.2 | 65 | - |
1 mM 4-hydroxybenzoate, 95% loss of activity | Moraxella sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.2 | 7 | 7.3 | reaction with NADH | Moraxella sp. |
1.14.13.2 | 7.5 | 7.8 | reaction with NADPH | Moraxella sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.2 | FAD | contains 0.12 mol FAD per mol of enzyme | Moraxella sp. | |
1.14.13.2 | NADH | activity with NADH is higher than activity with NADPH | Moraxella sp. | |
1.14.13.2 | NADPH | activity with NADH is higher than activity with NADPH | Moraxella sp. |