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Literature summary extracted from

  • Sterjiades, R.
    Properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Moraxella sp (1993), Biotechnol. Appl. Biochem., 17, 77-90.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.2 3-hydroxybenzoate 2 mM, 26% inhibition Moraxella sp.
1.14.13.2 4-hydroxy-3-nitrobenzoic acid 2 mM, 23% inhibition Moraxella sp.
1.14.13.2 4-hydroxybenzaldehyde 2 mM, 27% inhibition Moraxella sp.
1.14.13.2 4-hydroxybenzoate above 0.1 mM Moraxella sp.
1.14.13.2 4-hydroxyphenylacetic acid 2 mM, 16% inhibition Moraxella sp.
1.14.13.2 4-nitrophenol 2 mM, 39% inhibition Moraxella sp.
1.14.13.2 benzoate 2 mM, 48% inhibition Moraxella sp.
1.14.13.2 coumaric acid 2 mM, 30% inhibition Moraxella sp.
1.14.13.2 Hg2+ 0.1 mM HgCl2, complete inhibition Moraxella sp.
1.14.13.2 PCMB 0.015 mM, more than 70% inhibition, partially restored by addition of 0.14 M 2-mercaptoethanol Moraxella sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.2 0.0095
-
4-hydroxybenzoate reaction with NADH Moraxella sp.
1.14.13.2 0.037
-
NADH
-
Moraxella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.2 45000
-
2 * 45000, SDS-PAGE Moraxella sp.
1.14.13.2 85000
-
gel filtration Moraxella sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADPH + O2 Moraxella sp. degradation of 4-hydroxybenzoate ?
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 Moraxella sp. GU2 degradation of 4-hydroxybenzoate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Moraxella sp.
-
-
-
1.14.13.2 Moraxella sp. GU2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.2
-
Moraxella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.2 10.2
-
-
Moraxella sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.2 -70°C, 5 mM potassium phosphate, pH 7.5, 5% glycerol, stable for several months Moraxella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
Moraxella sp. protocatechuate + NAD+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
Moraxella sp. GU2 protocatechuate + NAD+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Moraxella sp. protocatechuate + NADP+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Moraxella sp. GU2 protocatechuate + NADP+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 degradation of 4-hydroxybenzoate Moraxella sp. ?
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 degradation of 4-hydroxybenzoate Moraxella sp. GU2 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.2 dimer 2 * 45000, SDS-PAGE Moraxella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.2 52
-
pH 7.5, 20 mM potassium phosphate buffer, 20 min, stable Moraxella sp.
1.14.13.2 60
-
in presence of 1 mM 4-hydroxybenzoate, 40% loss of activity after 20 min Moraxella sp.
1.14.13.2 65
-
1 mM 4-hydroxybenzoate, 95% loss of activity Moraxella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.2 7 7.3 reaction with NADH Moraxella sp.
1.14.13.2 7.5 7.8 reaction with NADPH Moraxella sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 FAD contains 0.12 mol FAD per mol of enzyme Moraxella sp.
1.14.13.2 NADH activity with NADH is higher than activity with NADPH Moraxella sp.
1.14.13.2 NADPH activity with NADH is higher than activity with NADPH Moraxella sp.