Any feedback?
Literature summary extracted from
- Hydroxybenzoate hydroxylase (1990), Methods Enzymol., 188, 138-147.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.2 | 5-Hydroxypicolinate | stimulates rapid oxidation of NADPH | Pseudomonas aeruginosa |  |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.14.13.2 | - |
Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.2 | 4-Aminosalicylate | competitive | Pseudomonas aeruginosa | |
| 1.14.13.2 | 6-Aminonicotinate | competitive | Pseudomonas aeruginosa | |
| 1.14.13.2 | 6-Hydroxynicotinate | - |
Pseudomonas aeruginosa | |
| 1.14.13.2 | Cl- | - |
Pseudomonas aeruginosa | |
| 1.14.13.2 | CNS- | - |
Pseudomonas aeruginosa | |
| 1.14.13.2 | I- | - |
Pseudomonas aeruginosa | |
| 1.14.13.2 | N3- | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.2 | 45100 | - |
2 * 45100 | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.2 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.2 | - |
Pseudomonas aeruginosa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.2 | 0°C-4°C, as a precipitate under a solution of 50 mM potassium phosphate and 0.5 mM EDTA, pH 6.5-7.0, with 70% saturated ammonium sulfate, idefinitely stable | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? | |
| 1.14.13.2 | 4-mercaptobenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | 4,4'-dithiobisbenzoate + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.2 | dimer | 2 * 45100 | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.2 | additional information | - |
additional information | - |
Pseudomonas aeruginosa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.2 | 1-deaza-FAD | when 1-deaza-FAD is used as cofactor, the enzyme carries out each step in catalysis except the transfer of oxygen to 4-hydroxybenzoate | Pseudomonas aeruginosa | |
| 1.14.13.2 | 6-Hydroxy-FAD | when 6-hydroxy-FAD is used as cofactor, the enzyme has a lower turnover rate than the native enzyme | Pseudomonas aeruginosa | |
| 1.14.13.2 | FAD | contains 1 mol of FAD per subunit | Pseudomonas aeruginosa | |
| 1.14.13.2 | NADPH | - |
Pseudomonas aeruginosa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
